Rhinoscleroma pathogenesis: the type K3 capsule of Klebsiella rhinoscleromatis is a virulence factor not involved in Mikulicz cells formation

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dc.contributor.authorCorelli, Barbara
dc.contributor.authorAlmeida, Ana S.
dc.contributor.authorSonego, Fabiane
dc.contributor.authorCastiglia, Virginia
dc.contributor.authorFevre, Cindy
dc.contributor.authorBrisse, Sylvain
dc.contributor.authorSansonetti, Philippe J.
dc.contributor.authorTournebize, Régis
dc.contributor.funderSeventh Framework Programme
dc.contributor.funderFondation pour la Recherche Médicale
dc.contributor.funderFundação para a Ciência e a Tecnologia
dc.contributor.funderAgence Nationale de la Recherche
dc.date.accessioned2018-03-09T12:55:07Z
dc.date.available2018-03-09T12:55:07Z
dc.date.issued2018
dc.description.abstractRhinoscleroma is a human specific chronic granulomatous infection of the nose and upper airways caused by the Gram-negative bacterium Klebsiella pneumoniae subsp. rhinoscleromatis. Although considered a rare disease, it is endemic in low-income countries where hygienic conditions are poor. A hallmark of this pathology is the appearance of atypical foamy monocytes called Mikulicz cells. However, the pathogenesis of rhinoscleroma remains poorly investigated. Capsule polysaccharide (CPS) is a prominent virulence factor in bacteria. All K. rhinoscleromatis strains are of K3 serotype, suggesting that CPS can be an important driver of rhinoscleroma disease. In this study, we describe the creation of the first mutant of K. rhinoscleromatis, inactivated in its capsule export machinery. Using a murine model recapitulating the formation of Mikulicz cells in lungs, we observed that a K. rhinoscleromatis CPS mutant (KR cps-) is strongly attenuated and that mice infected with a high dose of KR cps- are still able to induce Mikulicz cells formation, unlike a K. pneumoniae capsule mutant, and to partially recapitulate the characteristic strong production of IL-10. Altogether, the results of this study show that CPS is a virulence factor of K. rhinoscleromatis not involved in the specific appearance of Mikulicz cells.en
dc.description.sponsorshipFundaçao para a Ciência e a Tecnologia (Investissement d'Avenir program Laboratoire d'Excellence "Integrative Biology of Emerging Infectious Diseases" ( ANR-10-LABX-62-IBEID); Agence Nationale de la Recherche (Infrastructure d’Avenir en Biologie et Santé "France Life Imaging" (ANR-11-INBS-0006); France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0006201
dc.identifier.citationCorelli, B., Almeida, A. S., Sonego, F., Castiglia, V., Fevre, C., Brisse, S., Sansonetti, P. J. and Tournebize, R. (2018) 'Rhinoscleroma pathogenesis: the type K3 capsule of Klebsiella rhinoscleromatis is a virulence factor not involved in Mikulicz cells formation', PLOS Neglected Tropical Diseases, 12(1), e0006201 (19pp). doi: 10.1371/journal.pntd.0006201en
dc.identifier.doi10.1371/journal.pntd.0006201
dc.identifier.endpage19
dc.identifier.issn1935-2727
dc.identifier.issn1935-2735
dc.identifier.issued1
dc.identifier.journaltitlePLOS Neglected Tropical Diseasesen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/5610
dc.identifier.volume12
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/316682/EU/Infection biology training network: shaping the future of infectious diseases treatments/INBIONET
dc.relation.urihttp://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006201
dc.rights© 2018, Corelli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectRhinoscleromaen
dc.subjectMikulicz cellsen
dc.subjectInfectionen
dc.subjectLungsen
dc.titleRhinoscleroma pathogenesis: the type K3 capsule of Klebsiella rhinoscleromatis is a virulence factor not involved in Mikulicz cells formationen
dc.typeArticle (peer-reviewed)en
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