The effect of sphingosine‐1‐phosphate on the endothelial glycocalyx during ischemia‐reperfusion injury in the isolated rat heart

Simple item page
dc.check.date2021-02-03
dc.check.infoAccess to this article is restricted until 12 months after publication by request of the publisher.en
dc.contributor.authorAraibi, Hala
dc.contributor.authorvan der Merwe, Elizabeth
dc.contributor.authorGwanyanya, Asfree
dc.contributor.authorKelly-Laubscher, Roisin
dc.contributor.funderSouth African Medical Research Councilen
dc.contributor.funderMinistry of Higher Education and Scientific Researchen
dc.contributor.funderNational Research Foundation of South Africaen
dc.date.accessioned2020-09-08T07:58:59Z
dc.date.available2020-09-08T07:58:59Z
dc.date.issued2020-02-03
dc.description.abstractObjective: Sphingosine‐1‐phosphate is a natural metabolite that is cardioprotective, but its effects on endothelial glycocalyx damage during ischemia‐reperfusion are unknown. Therefore, we investigated the effect of sphingosine‐1‐phosphate on the endothelial glycocalyx during ischemia‐reperfusion. Methods: Isolated hearts from Wistar rats were perfused on a Langendorff system with Krebs‐Henseleit buffer and pretreated with sphingosine‐1‐phosphate (10 nmol/L) before ischemia‐reperfusion. Infarct size was measured by triphenyl tetrazolium chloride staining (n ≥ 6 per group). Cardiac edema was assessed by calculating total water content (n = 7 per group) and histologically quantifying the interstitial compartment (n ≥ 3 per group). The post‐ischemic coronary release of syndecan‐1 was quantified using ELISA. Syndecan‐1 immunostaining intensity was assessed in perfusion‐fixed hearts (n ≥ 3 per group). Results: Pretreatment with sphingosine‐1‐phosphate decreased infarct size in isolated hearts subjected to ischemia‐reperfusion (P = .01 vs ischemia‐reperfusion). However, sphingosine‐1‐phosphate had no effect on syndecan‐1 levels in the coronary effluent or on the intensity of the syndecan‐1 immunostaining signal in cardiac tissue. Heart total water content was not significantly different between control and ischemic groups but was significantly decreased in hearts treated with sphingosine‐1‐phosphate alone. Conclusion: These results suggest that sphingosine‐1‐phosphate‐induced cardioprotection against ischemia‐reperfusion injury is not mediated by the maintenance of syndecan‐1 in the endothelial glycocalyx.en
dc.description.sponsorshipSouth African Medical Research Council (Grant Number: 29841); National Research Foundation (Grant Number: 85768)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide12612en
dc.identifier.citationAraibi, H., van der Merwe, E., Gwanyanya, A. and Kelly‐Laubscher, R. (2020) 'The effect of sphingosine‐1‐phosphate on the endothelial glycocalyx during ischemia‐reperfusion injury in the isolated rat heart', Microcirculation, 27(5), e12612 (11pp). doi: 10.1111/micc.12612en
dc.identifier.doi10.1111/micc.12612en
dc.identifier.eissn1549-8719
dc.identifier.endpage11en
dc.identifier.issn1073-9688
dc.identifier.issued5en
dc.identifier.journaltitleMicrocirculationen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/10482
dc.identifier.volume27en
dc.language.isoenen
dc.publisherJohn Wiley & Sons, Inc.en
dc.rights© 2020, John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Araibi, H., van der Merwe, E., Gwanyanya, A. and Kelly‐Laubscher, R. (2020) 'The effect of sphingosine‐1‐phosphate on the endothelial glycocalyx during ischemia‐reperfusion injury in the isolated rat heart', Microcirculation, 27(5), e12612 (11pp), doi: 10.1111/micc.12612, which has been published in final form at https://doi.org/10.1111/micc.12612. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en
dc.subjectCardiac edemaen
dc.subjectGlycocalyxen
dc.subjectIschemia‐reperfusionen
dc.subjectMyocardial infarctionen
dc.subjectS1Pen
dc.titleThe effect of sphingosine‐1‐phosphate on the endothelial glycocalyx during ischemia‐reperfusion injury in the isolated rat hearten
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
edited paper for microcirculation final Jan 2020.pdf
Size:
9.05 MB
Format:
Adobe Portable Document Format
Description:
Accepted Version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pharmacology and Therapeutics - Journal Articles