Functional characterization of the Lactolisterin BU gene cluster of Lactococcus lactis subsp. lactis BGBU1-4

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dc.contributor.authorMiljkovic, Marija
dc.contributor.authorLozo, Jelena
dc.contributor.authorMirkovic, Nemanja
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorMalesevic, Milka
dc.contributor.authorJovcic, Branko
dc.contributor.authorCotter, Paul D.
dc.contributor.authorKojic, Milan
dc.contributor.funderMinistarstvo Prosvete, Nauke i Tehnološkog Razvojaen
dc.date.accessioned2019-09-04T08:17:12Z
dc.date.available2019-09-04T08:17:12Z
dc.date.issued2018-11-15
dc.description.abstractThe gene cluster responsible for the production of the aureocin A53-like bacteriocin, lactolisterin BU, is located on plasmid pBU6 in Lactococcus lactis subsp. lactis BGBU1- 4. Heterologous expression of pBU6 confirmed that production and limited immunity to lactolisterin BU were provided by the plasmid. Comparative analysis of aureocin A53- like operons revealed that the structural genes shared a low level of identity, while other genes were without homology, indicating a different origin. Subcloning and expression of genes located downstream of the structural gene, lliBU, revealed that the lactolisterin BU cluster consists of four genes: the structural gene lliBU, the abcT gene encoding an ABC transporter, the accL gene encoding an accessory protein and the immL gene which provides limited immunity to lactolisterin BU. Reverse transcription analysis revealed that all genes were transcribed as one polycistronic mRNA. Attempts to split the lactolisterin BU operon, even when both parts were under control of the PlliBU promoter, were unsuccessful indicate g that expression of lactolisterin BU is probably precisely regulated at the translational level by translational coupling and is possible only when all genes of the operon are in cis constellation. Two ρ-independent transcription terminators were detected in the lactolisterin BU operon: the first in the intergenic region of the lliBU and abcT genes and the second at the end of operon. Deletion of the second transcription terminator did not influence production of the bacteriocin in lactococci.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid2774en
dc.identifier.citationMiljković, M., Lozo, J., Mirkovic, N., O'Connor, P.M., Malesevic, M., Jovčić, B., Cotter, P.D. and Kojic, M., 2018. Functional Characterization of the Lactolisterin BU Gene Cluster of Lactococcus lactis subsp. lactis BGBU1-4. Frontiers in microbiology, 9, (2774). DOI: 10.3389/fmicb.2018.02774en
dc.identifier.doi10.3389/fmicb.2018.02774en
dc.identifier.eissn1664-302X
dc.identifier.endpage15en
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8444
dc.identifier.volume9en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.projectinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS/Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan/en
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2018.02774/full
dc.rights© 2018 Miljkovic, Lozo, Mirkovic, O’Connor, Malesevic, Jovcic, Cotter and Kojicen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriocinen
dc.subjectLactolisterin BU operonen
dc.subjectABC transporteren
dc.subjectLimited immuniten
dc.subjectTranslational couplingen
dc.titleFunctional characterization of the Lactolisterin BU gene cluster of Lactococcus lactis subsp. lactis BGBU1-4en
dc.typeArticle (peer-reviewed)en
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