Myosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection
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Published Version
Date
2011-02-17
Authors
Rosberg-Cody, Eva
Liavonchanka, Alena
Göbel, Cornelia
Ross, R. Paul
O'Sullivan, Orla
Fitzgerald, Gerald F.
Feussner, Ivo
Stanton, Catherine
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Published Version
Abstract
Background: The aim of this study was to determine the catalytic activity and physiological role of myosin-crossreactive antigen (MCRA) from Bifidobacterium breve NCIMB 702258. MCRA from B. breve NCIMB 702258 was cloned,
sequenced and expressed in heterologous hosts (Lactococcus and Corynebacterium) and the recombinant proteins
assessed for enzymatic activity against fatty acid substrates.
Results: MCRA catalysed the conversion of palmitoleic, oleic and linoleic acids to the corresponding 10-hydroxy
fatty acids, but shorter chain fatty acids were not used as substrates, while the presence of trans-double bonds and
double bonds beyond the position C12 abolished hydratase activity. The hydroxy fatty acids produced were not
metabolised further. We also found that heterologous Lactococcus and Corynebacterium expressing MCRA
accumulated increasing amounts of 10-HOA and 10-HOE in the culture medium. Furthermore, the heterologous
cultures exhibited less sensitivity to heat and solvent stresses compared to corresponding controls.
Conclusions: MCRA protein in B. breve can be classified as a FAD-containing double bond hydratase, within the
carbon-oxygen lyase family, which may be catalysing the first step in conjugated linoleic acid (CLA) production,
and this protein has an additional function in bacterial stress protection.
Description
Keywords
Myosin-cross-reactive antigen (MCRA) , Bifidobacterium breve , Fatty acid hydratase , Stress protection , Lactococcus , Corynebacterium
Citation
Rosberg-Cody, E., Liavonchanka, A., Göbel, C., Ross, R.P., O'Sullivan, O., Fitzgerald, G.F., Feussner, I., Stanton, C., 2011. Myosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection. BMC Biochemistry 12(9). doi: 10.1186/1471-2091-12-9