Lysogenization of a lactococcal host with three distinct temperate phages provides homologous and heterologous phage resistance

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dc.contributor.authorRuiz-Cruz, Sofiaen
dc.contributor.authorParlindungan, Elvinaen
dc.contributor.authorErazo Garzon, Andreaen
dc.contributor.authorAlqarni, Monaen
dc.contributor.authorLugli, Gabriele A.en
dc.contributor.authorVentura, Marcoen
dc.contributor.authorvan Sinderen, Douween
dc.contributor.authorMahony, Jenniferen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2023-11-14T15:26:30Z
dc.date.available2023-11-14T15:26:30Z
dc.date.issued2020en
dc.description.abstractLactococcus lactis is the most widely exploited microorganism in global dairy fermentations. Lactococcal strains are described as typically harboring a number of prophages in their chromosomes. The presence of such prophages may provide both advantages and disadvantages to the carrying host. Here, we describe the deliberate generation of three distinct lysogens of the model lactococcal strain 3107 and the impact of additional prophage carriage on phage-resistance and anti-microbial susceptibility. Lysogen-specific responses were observed, highlighting the unique relationship and impact of each lysogenic phage on its host. Both homologous and heterologous phage-resistance profiles were observed, highlighting the presence of possible prophage-encoded phage-resistance factors. Superinfection exclusion was among the most notable causes of heterologous phage-resistance profiles with resistance observed against members of the Skunavirus, P335, P087, and 949 lactococcal phage groups. Through these analyses, it is now possible to identify phages that may pursue similar DNA injection pathways. The generated lysogenic strains exhibited increased sensitivity to the antimicrobial compounds, nisin and lysozyme, relative to the parent strain, although it is noteworthy that the degree of sensitivity was specific to the individual (pro)phages. Overall, the findings highlight the unique impact of each prophage on a given strain and the requirement for strain-level analysis when considering the implications of lysogeny.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1685en
dc.identifier.citationRuiz-Cruz, S., Parlindungan, E., Erazo Garzon, A., Alqarni, M., Lugli, G.A., Ventura, M., Van Sinderen, D. and Mahony, J. (2020) ‘Lysogenization of a lactococcal host with three distinct temperate phages provides homologous and heterologous phage resistance’, Microorganisms, 8(11), 1685 (14pp). doi: 10.3390/microorganisms8111685en
dc.identifier.doi10.3390/microorganisms8111685en
dc.identifier.endpage14en
dc.identifier.issn2076-2607en
dc.identifier.issued11en
dc.identifier.journaltitleMicroorganismsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15228
dc.identifier.volume8en
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.ispartofMicroorganismsen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriophageen
dc.subjectLactococcus lactisen
dc.subjectPhage-resistanceen
dc.subjectAbortive infectionen
dc.titleLysogenization of a lactococcal host with three distinct temperate phages provides homologous and heterologous phage resistanceen
dc.typeArticle (peer-reviewed)en
dc.typejournal-articleen
oaire.citation.issue11en
oaire.citation.volume8en
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