Overview of optical and electrochemical alkaline phosphatase (ALP) biosensors: recent approaches in cells culture techniques

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dc.contributor.authorBalbaied, Thanih
dc.contributor.authorMoore, Eric
dc.contributor.funderMinistry of Education – Kingdom of Saudi Arabien
dc.date.accessioned2019-10-06T21:53:56Z
dc.date.available2019-10-06T21:53:56Z
dc.date.issued2019-08-23
dc.description.abstractAlkaline phosphatase (ALP), which catalyzes the dephosphorylation process of proteins, nucleic acids, and small molecules, can be found in a variety of tissues (intestine, liver, bone, kidney, and placenta) of almost all living organisms. This enzyme has been extensively used as a biomarker in enzyme immunoassays and molecular biology. ALP is also one of the most commonly assayed enzymes in routine clinical practice. Due to its close relation to a variety of pathological processes, ALP’s abnormal level is an important diagnostic biomarker of many human diseases, such as liver dysfunction, bone diseases, kidney acute injury, and cancer. Therefore, the development of convenient and reliable assay methods for monitoring ALP activity/level is extremely important and valuable, not only for clinical diagnoses but also in the area of biomedical research. This paper comprehensively reviews the strategies of optical and electrochemical detection of ALP and discusses the electrochemical techniques that have been addressed to make them suitable for ALP analysis in cell culture.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid102en
dc.identifier.citationBalbaied, T. and Moore, E. (2019) 'Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques', Biosensors, 9(3), 102 (30pp). DOI: 10.3390/bios9030102en
dc.identifier.doi10.3390/bios9030102en
dc.identifier.eissn2079-6374
dc.identifier.endpage30en
dc.identifier.issued3en
dc.identifier.journaltitleBiosensorsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8714
dc.identifier.volume9en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttps://www.mdpi.com/2079-6374/9/3/102/htm
dc.rights©2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAlkaline phosphatase (ALP)en
dc.subjectOptical biosensorsen
dc.subjectElectrochemical biosensorsen
dc.subjectNanomaterialsen
dc.subjectMicroarrays technologyen
dc.subjectLab-on-chip technologyen
dc.titleOverview of optical and electrochemical alkaline phosphatase (ALP) biosensors: recent approaches in cells culture techniquesen
dc.typeArticle (peer-reviewed)en
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