Effects of intracerebroventricular leptin and orexin-A on the baroreflex control of renal sympathetic nerve activity in conscious rats fed a normal or high-fat diet

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dc.check.date2021-12-22
dc.check.infoAccess to this article is restricted until 12 months after publication by request of the publisher.en
dc.contributor.authorHuang, Chunlong
dc.contributor.authorAlMarabeh, Sara
dc.contributor.authorCavers, Jeremy
dc.contributor.authorAbdulla, Mohammed H.
dc.contributor.authorJohns, Edward J.
dc.contributor.funderHealth Research Boarden
dc.date.accessioned2021-01-21T12:12:21Z
dc.date.available2021-01-21T12:12:21Z
dc.date.issued2020-12-22
dc.date.updated2021-01-21T11:59:27Z
dc.description.abstractThis study examined the effect of leptin and orexin‐A on autonomic baroreflex control in conscious Wistar rats exposed to high‐fat (45% fat) or normal (3.4%) diet for 4 weeks. Renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP) and heart rate (HR) were monitored during the generation of baroreflex gain curves and acute volume expansion (VEP). Intracerebroventricular (ICV) leptin (1 μg/min) increased RSNA in the normal diet group (0.31 ± 0.04 vs 0.23 ± 0.03 mV/s) and MAP in the high‐fat diet group (115 ± 5 vs 105 ± 5 mm Hg, P < .05). Orexin‐A (50 ng/min) increased RSNA, HR and MAP in the high‐fat diet group (0.26 ± 0.03 vs 0.22 ± 0.02 mV/s, 454 ± 8 vs 417 ± 12 beats/min, 117 ± 1 vs 108 ± 1 mm Hg) and the normal diet group (0.18 ± 0.05 vs 0.17 ± 0.05 mV/s, 465 ± 10 vs 426 ± 6 beats/min, 116 ± 2 vs 104 ± 3 mm Hg). Baroreflex sensitivity for RSNA was increased during ICV leptin by 50% in the normal diet group, compared to 14% in the high‐fat diet group (P < .05). Similarly, orexin‐A increased baroreflex sensitivity by 56% and 50% in the high‐fat and normal diet groups, respectively (all P < .05). During ICV saline, VEP decreased RSNA by 31 ± 5% (P < .05) after 10 minutes and the magnitude of this response was blunted during ICV infusion of leptin (17 ± 2%, P < .05) but not orexin‐A in the normal diet group. RSNA response to VEP was not changed during ICV leptin or orexin‐A in the high‐fat diet group. These findings indicate possible central roles for leptin and orexin‐A in modulating the baroreflexes under normal or increased fat intake in conscious rats and potential therapeutic approaches for obesity associated hypertension.en
dc.description.sponsorshipHealth Research Board (Grant Number: RP/2007/17)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationHuang, C., AlMarabeh, S., Cavers, J., Abdulla, M. H. and Johns, E. J. (2020) 'Effects of intracerebroventricular leptin and orexin-A on the baroreflex control of renal sympathetic nerve activity in conscious rats fed a normal or high-fat diet', Clinical and Experimental Pharmacology and Physiology. doi: 10.1111/1440-1681.13451en
dc.identifier.doi10.1111/1440-1681.13451en
dc.identifier.eissn1440-1681
dc.identifier.issn0305-1870
dc.identifier.journaltitleClinical and Experimental Pharmacology and Physiologyen
dc.identifier.urihttps://hdl.handle.net/10468/10945
dc.language.isoenen
dc.publisherJohn Wiley & Sons, Inc.en
dc.rights© 2020, John Wiley & Sons Australia, Ltd. This is the peer reviewed version of the following article: Huang, C., AlMarabeh, S., Cavers, J., Abdulla, M. H. and Johns, E. J. (2020) 'Effects of intracerebroventricular leptin and orexin-A on the baroreflex control of renal sympathetic nerve activity in conscious rats fed a normal or high-fat diet', Clinical and Experimental Pharmacology and Physiology, doi: 10.1111/1440-1681.13451, which has been published in final form at https://doi.org/10.1111/1440-1681.13451. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en
dc.subjectBaroreflexen
dc.subjectHypertensionen
dc.subjectLeptinen
dc.subjectVolume expansionen
dc.subjectHigh-fat dieten
dc.subjectOrexin-Aen
dc.titleEffects of intracerebroventricular leptin and orexin-A on the baroreflex control of renal sympathetic nerve activity in conscious rats fed a normal or high-fat dieten
dc.typeArticle (peer-reviewed)en
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