Exposure of Bifidobacterium longum subsp. infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response

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Kavanaugh, Devon W.
O'Callaghan, John
Butto, Ludovica F.
Slattery, Helen
Lane, Jonathan
Clyne, Marguerite
Kane, Marian
Joshi, Lokesh
Hickey, Rita M.
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In this study, we tested the hypothesis that milk oligosaccharides may contribute not only to selective growth of bifidobacteria, but also to their specific adhesive ability. Human milk oligosaccharides (39sialyllactose and 69sialyllactose) and a commercial prebiotic (Beneo Orafti P95; oligofructose) were assayed for their ability to promote adhesion of Bifidobacterium longum subsp. infantis ATCC 15697 to HT-29 and Caco-2 human intestinal cells. Treatment with the commercial prebiotic or 39sialyllactose did not enhance adhesion. However, treatment with 69sialyllactose resulted in increased adhesion (4.7 fold), while treatment with a mixture of 39- and 69-sialyllactose substantially increased adhesion (9.8 fold) to HT-29 intestinal cells. Microarray analyses were subsequently employed to investigate the transcriptional response of B. longum subsp. infantis to the different oligosaccharide treatments. This data correlated strongly with the observed changes in adhesion to HT-29 cells. The combination of 39- and 69-sialyllactose resulted in the greatest response at the genetic level (both in diversity and magnitude) followed by 69sialyllactose, and 39sialyllactose alone. The microarray data was further validated by means of real-time PCR. The current findings suggest that the increased adherence phenotype of Bifidobacterium longum subsp. infantis resulting from exposure to milk oligosaccharides is multi-faceted, involving transcription factors, chaperone proteins, adhesion-related proteins, and a glycoside hydrolase. This study gives additional insight into the role of milk oligosaccharides within the human intestine and the molecular mechanisms underpinning hostmicrobe interactions.
Human milk oligosaccharides , Bacterial adhesion , Bifidobacterium longum
Kavanaugh DW, O’Callaghan J, Butto´ LF, Slattery H, Lane J, et al. (2013) Exposure of Bifidobacterium longum subsp. infantis to Milk Oligosaccharides Increases Adhesion to Epithelial Cells and Induces a Substantial Transcriptional Response. PLoS ONE 8(6): e67224. doi:10.1371/journal.pone.0067224