HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters

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dc.contributor.authorChoudhary, Alka
dc.contributor.authorNaughton, Lynn M.
dc.contributor.authorDobson, Alan D. W.
dc.contributor.authorRai, Dilip K.
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.date.accessioned2018-07-11T10:37:42Z
dc.date.available2018-07-11T10:37:42Z
dc.date.issued2018-07-03
dc.date.updated2018-07-11T10:15:57Z
dc.description.abstractRationale: In recent years, metabolites produced by Pseudovibrio species have gained scientific attention due to their potent antimicrobial activity. Recently we also have assessed antibacterial activities of Pseudovibrio sp. W64 isolates against Staphylococcus aureus, where only the dominant tropodithietic acid (TDA) was identified. However characterisation of other metabolites is necessary as these metabolites may also serve as potent antimicrobial agents. Methods: LC-MS/MS, aided by accurate mass measurements, was employed to screen and characterise a range of metabolites produced by Pseudovibrio sp. W64 via assessment of ethyl acetate fractions generated from bacterial cultures. Results: Thirteen metabolites unique to the bacterial culture were detected and their chemical structures were assigned by tandem mass spectrometry and accurate mass measurement. Among the thirteen metabolites, a methyl ester of TDA, a number of cholic acid derivatives, and amino diols and triols were characterised. Conclusions: Pseudovibrio sp. W64 produces methylated TDA in addition to TDA, and metabolises lipids and amino acids in the cell-culture medium. To the best of our knowledge, this is the first report of methylated TDA, cholic acid and its various analogs, and sphinganine being detected in this Pseudovibrio strain. The data generated may help to better understand the biochemical processes and metabolism of bacterial strains towards discovery of antimicrobial agents from marine sources.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine (FIRM 11/F/009)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationChoudhary, A., Naughton, L. M., Dobson, A. D.W. and Rai, D. K. (2018) 'HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters', Rapid Communications in Mass Spectrometry. doi:10.1002/rcm.8226en
dc.identifier.doi10.1002/rcm.8226
dc.identifier.issn0951-4198
dc.identifier.issn1097-0231
dc.identifier.journaltitleRapid Communications in Mass Spectrometryen
dc.identifier.urihttps://hdl.handle.net/10468/6451
dc.language.isoenen
dc.publisherJohn Wiley & Sons, Inc.en
dc.rights© 2018, John Wiley & Sons Inc. This is the peer reviewed version of the following article: Choudhary, A., Naughton, L. M., Dobson, A. D.W. and Rai, D. K. (2018) 'HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters', Rapid Communications in Mass Spectrometry. doi:10.1002/rcm.8226, which has been published in final form at https://doi.org/10.1002/rcm.8226. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en
dc.subjectPseudovibrio sp.en
dc.subjectTropodithietic aciden
dc.subjectCholic aciden
dc.subjectSphinganineen
dc.subjectHPLC-MS/MSen
dc.titleHPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish watersen
dc.typeArticle (peer-reviewed)en
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