Uncovering bifidobacteria via targeted sequencing of the mammalian gut microbiota

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dc.contributor.authorLugli, Gabriele Andrea
dc.contributor.authorDuranti, Sabrina
dc.contributor.authorMilani, Christian
dc.contributor.authorMancabelli, Leonardo
dc.contributor.authorTurroni, Francesca
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorVentura, Marco
dc.contributor.funderEuropean Commissionen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-12-09T12:37:13Z
dc.date.available2019-12-09T12:37:13Z
dc.date.issued2019-11-06
dc.description.abstractBifidobacteria are among the most prevalent gut commensals in mammals, playing crucial functional roles that start from their early colonization of the infant gastrointestinal tract and last throughout the life span of their host. Metagenomic approaches have been employed to unveil the genetic features of bifidobacteria in order to understand how they participate in the correct development of a healthy microbiome. Nevertheless, their low relative abundance in many environmental samples may represent a major limitation for metagenomics approaches. To overcome this restriction, we applied an enrichment method that allows amplification of bifidobacterial DNA obtained from human or animal fecal samples for up to 26,500-fold, resulting in the metagenomic reconstruction of genomes belonging to bifidobacterial strains, present at very low abundance in collected samples. Functional predictions of the genes from these reconstructed genomes allows us to identify unique signatures among members of the same bifidobacterial species, highlighting genes correlated with the uptake of nutrients and adhesion to the intestinal mucosa.en
dc.description.sponsorshipScience Fondation Ireland (Grant number 15/JP-HDHL/3280, SFI/12/RC/2273a and SFI/12/RC/2273b)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid535en
dc.identifier.citationLugli, G. A., Duranti, S., Milani, C., Mancabelli, L., Turroni, F., van Sinderen, D. and Ventura, M. (2019) 'Uncovering Bifidobacteria via Targeted Sequencing of the Mammalian Gut Microbiota', Microorganisms, 7(11), 535 (11pp). doi: 10.3390/microorganisms7110535en
dc.identifier.doi10.3390/microorganisms7110535en
dc.identifier.eissn2076-2607
dc.identifier.endpage11en
dc.identifier.issued11en
dc.identifier.journaltitleMicroorganismsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9365
dc.identifier.volume7en
dc.language.isoenen
dc.publisherMDPI AGen
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectGenomicsen
dc.subjectMetagenomicsen
dc.subjectMicrobiotaen
dc.subjectBifidobacteriumen
dc.titleUncovering bifidobacteria via targeted sequencing of the mammalian gut microbiotaen
dc.typeArticle (peer-reviewed)en
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