Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium

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dc.contributor.authorEgan, Muireann
dc.contributor.authorO'Connell Motherway, Mary
dc.contributor.authorKilcoyne, Michelle
dc.contributor.authorKane, Marian
dc.contributor.authorJoshi, Lokesh
dc.contributor.authorVentura, Marco
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderHealth Research Boarden
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Commissionen
dc.date.accessioned2016-01-20T10:41:57Z
dc.date.available2016-01-20T10:41:57Z
dc.date.issued2014-11-25
dc.description.abstractBACKGROUND: Bifidobacteria constitute a specific group of commensal bacteria that commonly inhabit the mammalian gastrointestinal tract. Bifidobacterium breve UCC2003 was previously shown to utilize a variety of plant/diet/host-derived carbohydrates, including cellodextrin, starch and galactan, as well as the mucin and HMO-derived monosaccharide, sialic acid. In the current study, we investigated the ability of this strain to utilize parts of a host-derived source of carbohydrate, namely the mucin glycoprotein, when grown in co-culture with the mucin-degrading Bifidobacterium bifidum PRL2010. RESULTS: B. breve UCC2003 was shown to exhibit growth properties in a mucin-based medium, but only when grown in the presence of B. bifidum PRL2010, which is known to metabolize mucin. A combination of HPAEC-PAD and transcriptome analyses identified some of the possible monosaccharides and oligosaccharides which support this enhanced co-cultivation growth/viability phenotype. CONCLUSION: This study describes the potential existence of a gut commensal relationship between two bifidobacterial species. We demonstrate the in vitro ability of B. breve UCC2003 to cross-feed on sugars released by the mucin-degrading activity of B. bifidum PRL2010, thus advancing our knowledge on the metabolic adaptability which allows the former strain to colonize the (infant) gut by its extensive metabolic abilities to (co-)utilize available carbohydrate sources.en
dc.description.sponsorshipScience Foundation Ireland ((SFI 07/CE/B1368), (SFI/12/RC/2273),( SFI 08/SRC/B1393)); European Commission (EU FP7 programme (Grant no. 260600)); Health Research Board (HRB postdoctoral fellowship (Grant No. PDTM/20011/9))en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid282
dc.identifier.citationEGAN, M., O’CONNELL MOTHERWAY, M., KILCOYNE, M., KANE, M., JOSHI, L., VENTURA, M. & VAN SINDEREN, D. 2014. Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium. BMC Microbiology, 14:282, 1-14. http://dx.doi.org/10.1186/s12866-014-0282-7en
dc.identifier.doi10.1186/s12866-014-0282-7
dc.identifier.endpage14en
dc.identifier.issn1471-2180
dc.identifier.journaltitleBMC Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/2198
dc.identifier.volume14en
dc.language.isoenen
dc.publisherBiomed Central Ltd.en
dc.rights© 2014 Egan et al.; licensee BioMed Central Ltd., 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBifidobacteriaen
dc.subjectProbioticen
dc.subjectFucoseen
dc.subjectSialic aciden
dc.subjectGalactoseen
dc.subjectHuman milken
dc.subjectOligosaccharidesen
dc.subjectHuman colon ecosystemsen
dc.subjectDNA microarray dataen
dc.subjectCarbohydrateen
dc.subjectAnaerobic bacteriaen
dc.subjectIntestinal tracten
dc.subjectGene expressionen
dc.subjectO-glycosylationen
dc.subjectGenome analysisen
dc.subjectLeloir pathwayen
dc.titleCross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based mediumen
dc.typeArticle (peer-reviewed)en
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