Characterization and induction of prophages in human gut-associated Bifidobacterium hosts

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dc.contributor.authorMavrich, Travis N.
dc.contributor.authorCasey, Eoghan
dc.contributor.authorOliveira, Joana
dc.contributor.authorBottacini, Francesca
dc.contributor.authorJames, Kieran
dc.contributor.authorFranz, Charles M. A. P.
dc.contributor.authorLugli, Gabriele Andrea
dc.contributor.authorNeve, Horst
dc.contributor.authorVentura, Marco
dc.contributor.authorHatfull, Graham F.
dc.contributor.authorMahony, Jennifer
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-11-25T10:40:55Z
dc.date.available2019-11-25T10:40:55Z
dc.date.issued2018-08-24
dc.description.abstractIn the current report, we describe the identification of three genetically distinct groups of prophages integrated into three different chromosomal sites of human gut-associated Bifidobacterium breve and Bifidobacterium longum strains. These bifidobacterial prophages are distantly related to temperate actinobacteriophages of several hosts. Some prophages, integrated within the dnaJ2 gene, are competent for induction, excision, replication, assembly and lysis, suggesting that they are fully functional and can generate infectious particles, even though permissive hosts have not yet been identified. Interestingly, several of these phages harbor a putative phase variation shufflon (the Rin system) that generates variation of the tail-associated receptor binding protein (RBP). Unlike the analogous coliphage-associated shufflon Min, or simpler Cin and Gin inversion systems, Rin is predicted to use a tyrosine recombinase to promote inversion, the first reported phage-encoded tyrosine-family DNA invertase. The identification of bifidobacterial prophages with RBP diversification systems that are competent for assembly and lysis, yet fail to propagate lytically under laboratory conditions, suggests dynamic evolution of bifidobacteria and their phages in the human gut.en
dc.description.sponsorshipGROW (12/RC/2273s3_GROW); SFI (15/JP-HDHL/3280); MIUR Italyen
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid12772en
dc.identifier.citationMavrich, T.N., Casey, E., Oliveira, J., Bottacini, F., James, K., Franz, C.M., Lugli, G.A., Neve, H., Ventura, M., Hatfull, G.F. and Mahony, J. (2018) 'Characterization and induction of prophages in human gut-associated Bifidobacterium hosts'. Scientific reports, 8(1), 12772. (17pp). doi:10.1038/s41598-018-31181-3en
dc.identifier.doi10.1038/s41598-018-31181-3en
dc.identifier.endpage17en
dc.identifier.issn2045-2322
dc.identifier.issued1en
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9216
dc.identifier.volume8en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.projectinfo:eu-repo/grantAgreement/NSF/Directorate for Education & Human Resources::Division of Graduate Education/1247842/US/Graduate Research Fellowship Program (GRFP)/en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::CSA/696300/EU/The second coordination and support action for the JPI Healthy Diet for a Healthy Life/CSA JPI HDHL 2.0en
dc.relation.urihttps://www.nature.com/articles/s41598-018-31181-3
dc.rights© The Author(s) 2018en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBifidobacterium breveen
dc.subjectBifidobacterium longumen
dc.subjectBifidobacterial prophagesen
dc.subjectActinobacteriophagesen
dc.subjectHuman guten
dc.subjectInfectious particlesen
dc.titleCharacterization and induction of prophages in human gut-associated Bifidobacterium hostsen
dc.typeArticle (peer-reviewed)en
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