Two groups of phenylalanine biosynthetic operon leader peptides genes: a high level of apparently incidental frameshifting in decoding Escherichia coli pheL

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dc.contributor.authorGurvich, Olga L.
dc.contributor.authorNäsvall, S. Joakim
dc.contributor.authorBaranov, Pavel V.
dc.contributor.authorBjörk, Glenn R.
dc.contributor.authorAtkins, John F.
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderNational Institutes of Health
dc.contributor.funderCarl Tryggers Stiftelse för Vetenskaplig Forskning
dc.contributor.funderSvenska Forskningsrådet Formas
dc.date.accessioned2017-11-14T13:24:31Z
dc.date.available2017-11-14T13:24:31Z
dc.date.issued2011
dc.description.abstractThe bacterial pheL gene encodes the leader peptide for the phenylalanine biosynthetic operon. Translation of pheL mRNA controls transcription attenuation and, consequently, expression of the downstream pheA gene. Fifty-three unique pheL genes have been identified in sequenced genomes of the gamma subdivision. There are two groups of pheL genes, both of which are short and contain a run(s) of phenylalanine codons at an inteRNAl position. One group is somewhat diverse and features different termination and 5'-flanking codons. The other group, mostly restricted to Enterobacteria and including Escherichia coli pheL, has a conserved nucleotide sequence that ends with UUC_CCC_UGA. When these three codons in E. coli pheL mRNA are in the ribosomal E-, P- and A-sites, there is an unusually high level, 15%, of +1 ribosomal frameshifting due to features of the nascent peptide sequence that include the penultimate phenylalanine. This level increases to 60% with a natural, heterologous, nascent peptide stimulator. Nevertheless, studies with different tRNA(Pro) mutants in Salmonella enterica suggest that frameshifting at the end of pheL does not influence expression of the downstream pheA. This finding of incidental, rather than utilized, frameshifting is cautionary for other studies of programmed frameshifting.en
dc.description.sponsorshipNational Institute of Health (GM079523)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGurvich, O. L., Näsvall, S. J., Baranov, P. V., Björk, G. R. and Atkins, J. F. (2011) 'Two groups of phenylalanine biosynthetic operon leader peptides genes: a high level of apparently incidental frameshifting in decoding Escherichia coli pheL', Nucleic Acids Research, 39(8), pp. 3079-3092. doi: 10.1093/nar/gkq1272en
dc.identifier.doi10.1093/nar/gkq1272
dc.identifier.endpage3092
dc.identifier.issn0305-1048
dc.identifier.issued8
dc.identifier.journaltitleNucleic Acids Researchen
dc.identifier.startpage3079
dc.identifier.urihttps://hdl.handle.net/10468/5025
dc.identifier.volume39
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttps://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkq1272
dc.rights© 2010, the Authors. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/2.5
dc.subjectTranslation terminationen
dc.subjectTransfer RNAen
dc.subjectSalmonella-typhimuriumen
dc.subjectNascent peptideen
dc.subjectMessenger RNAen
dc.subjectAttenuation regulationen
dc.subjectCoding gapen
dc.subjectIn-vivoen
dc.subjectRibosomeen
dc.subjectExpressionen
dc.titleTwo groups of phenylalanine biosynthetic operon leader peptides genes: a high level of apparently incidental frameshifting in decoding Escherichia coli pheLen
dc.typeArticle (peer-reviewed)en
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