Infant-associated bifidobacterial β-galactosidases and their ability to synthesize galacto-oligosaccharides

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dc.contributor.authorAmbrogi, Valentinaen
dc.contributor.authorBottacini, Francescaen
dc.contributor.authorO’Callaghan, Johnen
dc.contributor.authorCasey, Eoghanen
dc.contributor.authorvan Breen, Justinen
dc.contributor.authorSchoemaker, Barryen
dc.contributor.authorCao, Linqiuen
dc.contributor.authorKuipers, Basen
dc.contributor.authorO’Connell Motherway, Maryen
dc.contributor.authorSchoterman, Margrieten
dc.contributor.authorvan Sinderen, Douween
dc.contributor.funderFrieslandCampinaen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderFederation of European Microbiological Societiesen
dc.contributor.funderHealth Research Boarden
dc.date.accessioned2023-11-28T11:36:44Z
dc.date.available2023-11-28T11:36:44Z
dc.date.issued2021en
dc.description.abstractGalacto-oligosaccharides (GOS) represent non-digestible glycans that are commercially produced by transgalactosylation of lactose, and that are widely used as functional food ingredients in prebiotic formulations, in particular in infant nutrition. GOS consumption has been reported to enhance growth of specific bacteria in the gut, in particular bifidobacteria, thereby supporting a balanced gut microbiota. In a previous study, we assessed the hydrolytic activity and substrate specificity of seventeen predicted β-galactosidases encoded by various species and strains of infant-associated bifidobacteria. In the current study, we further characterized seven out of these seventeen bifidobacterial β-galactosidases in terms of their kinetics, enzyme stability and oligomeric state. Accordingly, we established whether these β-galactosidases are capable of synthesizing GOS via enzymatic transgalactosylation employing lactose as the feed substrate. Our findings show that the seven selected enzymes all possess such transgalactosylation activity, though they appear to differ in their efficiency by which they perform this reaction. From chromatography analysis, it seems that these enzymes generate two distinct GOS mixtures: GOS with a relatively short or long degree of polymerization profile. These findings may be the stepping stone for further studies aimed at synthesizing new GOS variants with novel and/or enhanced prebiotic activities and potential for industrial applications.en
dc.description.sponsorshipFederation of European Microbiological Societies (Research Grant FEMS-RG-2016-0103); Health Research Board (Grant No. 513 PDTM/20011/9)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid662959en
dc.identifier.citationAmbrogi, V., Bottacini, F., O’Callaghan, J., Casey, E., Van Breen, J., Schoemaker, B., Cao, L., Kuipers, B., O’Connell Motherway, M., Schoterman, M. and Van Sinderen, D. (2021) ‘Infant-associated bifidobacterial β-galactosidases and their ability to synthesize galacto-oligosaccharides’, Frontiers in Microbiology, 12, 662959 (12pp). doi: 10.3389/fmicb.2021.662959en
dc.identifier.doi10.3389/fmicb.2021.662959en
dc.identifier.endpage12en
dc.identifier.issn1664-302Xen
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15264
dc.identifier.volume12en
dc.language.isoenen
dc.publisherFrontiers Media S.A.en
dc.relation.ispartofFrontiers in Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2021 Ambrogi, Bottacini, O’Callaghan, Casey, van Breen, Schoemaker, Cao, Kuipers, O’Connell Motherway, Schoterman and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectPrebioticsen
dc.subjectGut microbiotaen
dc.subjectMicrobiomeen
dc.subjectBifidogenicen
dc.subjectGalacto-oligosaccharidesen
dc.subjectInfanten
dc.subjectOligosaccharidesen
dc.subjectBifidobacteriumen
dc.titleInfant-associated bifidobacterial β-galactosidases and their ability to synthesize galacto-oligosaccharidesen
dc.typeArticle (peer-reviewed)en
dc.typejournal-articleen
oaire.citation.volume12en
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