Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates

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dc.contributor.authorAmbrogi, Valentina
dc.contributor.authorBottacini, Francesca
dc.contributor.authorO'Sullivan, Joyce
dc.contributor.authorO'Connell Motherway, Mary
dc.contributor.authorLinqiu, Cao
dc.contributor.authorSchoemaker, Barry
dc.contributor.authorSchoterman, Margriet
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderFederation of European Microbiological Society (FEMS)en
dc.contributor.funderHealth Research Boarden
dc.contributor.funderFrieslandCampina, Netherlandsen
dc.date.accessioned2019-11-23T06:56:08Z
dc.date.available2019-11-23T06:56:08Z
dc.date.issued2019-01-19
dc.description.abstractBifidobacteria are among the first and most abundant bacterial colonizers of the gastrointestinal tract of (breast-fed) healthy infants. Their success of colonising the infant gut is believed to be, at least partly, due to their ability to metabolize available carbon sources by means of secreted or intracellular glycosyl hydrolases (GHs). Among these, β-galactosidases are particularly relevant as they allow bifidobacteria to grow on β-galactosyl-linked saccharidic substrates, which are present in copious amounts in the milk-based diet of their infant host (e.g. lactose and human milk oligosaccharides). In the present study we employed an in silico analysis to identify GH family 2 and 42 β-galactosidases encoded by typical infant-associated bifidobacteria. Comparative genome analysis followed by characterisation of selected β-galactosidases revealed how these GH2 and GH42 members are distributed among these infant-associated bifidobacteria, while their hydrolytic activity towards growth substrates commonly available in the infant gut were also assessed.en
dc.description.sponsorshipFEMS (RG-2016-0103); Health Research Board (513 PDTM/20011/9)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid9en
dc.identifier.citationAmbrogi, V., Bottacini, F., O’Sullivan, J., Motherway, M.O.C., Linqiu, C., Schoemaker, B., Schoterman, M. and van Sinderen, D. (2019) 'Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates' AMB Express, 9(1), 9. (15pp). doi:10.1186/s13568-019-0735-3en
dc.identifier.doi10.1186/s13568-019-0735-3en
dc.identifier.eissn2191-0855
dc.identifier.endpage15en
dc.identifier.issued1en
dc.identifier.journaltitleAMB Expressen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9200
dc.identifier.volume9en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.urihttps://amb-express.springeropen.com/articles/10.1186/s13568-019-0735-3
dc.rights© The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectβ-Galactosidasesen
dc.subjectBifidobacteriaen
dc.subjectLactoseen
dc.subjectHMOsen
dc.subjectInfant gut microbiotaen
dc.titleCharacterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolatesen
dc.typeArticle (peer-reviewed)en
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