Isolation and characterization of Lactobacillus brevis phages

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dc.contributor.authorFeyereisen, Marine
dc.contributor.authorMahony, Jennifer
dc.contributor.authorLugli, Gabriele A.
dc.contributor.authorVentura, Marco
dc.contributor.authorNeve, Horst
dc.contributor.authorFranz, Charles M. A. P.
dc.contributor.authorNoben, Jean-Paul
dc.contributor.authorO'Sullivan, Tadhg
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderIrish Research Councilen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-11-20T05:22:40Z
dc.date.available2019-11-20T05:22:40Z
dc.date.issued2019-04-26
dc.description.abstractLactobacillus brevis has been widely used in industry for fermentation purposes. However, it is also associated with the spoilage of foods and beverages, in particular, beer. There is an increasing demand for natural food preservation methods, and in this context, bacteriophages possess the potential to control such spoilage bacteria. Just a few studies on phages infecting Lactobacillus brevis have been performed to date and in the present study, we report the isolation and characterization of five virulent phages capable of infecting Lb. brevis strains. The analysis reveals a high diversity among the isolates, with members belonging to both, the Myoviridae and Siphoviridae families. One isolate, designated phage 3-521, possesses a genome of 140.8 kb, thus representing the largest Lb. brevis phage genome sequenced to date. While the isolated phages do not propagate on Lb. brevis beer-spoiling strains, phages showed activity against these strains, impairing the growth of some Lb. brevis strains. The results highlight the potential of bacteriophage-based treatments as an effective approach to prevent bacterial spoilage of beer.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid393en
dc.identifier.citationFeyereisen, M., Mahony, J., Lugli, G.A., Ventura, M., Neve, H., Franz, C.M., Noben, J.P., O’Sullivan, T. and Sinderen, D.V., 2019. Isolation and Characterization of Lactobacillus brevis Phages. Viruses, 11(5), (393). DOI:10.3390/v11050393en
dc.identifier.doi10.3390/v11050393en
dc.identifier.endpage16en
dc.identifier.issn1999-4915
dc.identifier.issued5en
dc.identifier.journaltitleVirusesen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9120
dc.identifier.volume11en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttps://www.mdpi.com/1999-4915/11/5/393
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerlanden
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectLactobacillus brevisen
dc.subjectBacteriophageen
dc.subjectVirulenten
dc.subjectBeeren
dc.subjectFermentationen
dc.subjectGenomic and proteomic analysisen
dc.titleIsolation and characterization of Lactobacillus brevis phagesen
dc.typeArticle (peer-reviewed)en
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