In silico prediction and exploration of potential bacteriocin gene clusters within the bacterial genus Geobacillus

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dc.contributor.authorEgan, Kevin
dc.contributor.authorField, Des
dc.contributor.authorRoss, R. Paul
dc.contributor.authorCotter, Paul D.
dc.contributor.authorHill, Colin
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-11-25T20:17:07Z
dc.date.available2019-11-25T20:17:07Z
dc.date.issued2018-09-20
dc.description.abstractThe thermophilic, endospore-forming genus of Geobacillus has historically been associated with spoilage of canned food. However, in recent years it has become the subject of much attention due its biotechnological potential in areas such as enzyme and biofuel applications. One aspect of this genus that has not been fully explored or realised is its use as a source of novel forms of the ribosomally synthesised antimicrobial peptides known as bacteriocins. To date only two bacteriocins have been fully characterised within this genus, i.e., Geobacillin I and II, with only a small number of others partially characterised. Here we bioinformatically investigate the potential of this genus as a source of novel bacteriocins through the use of the in silico screening software BAGEL3, which scans publically available genomes for potential bacteriocin gene clusters. In this study we examined the association of bacteriocin gene presence with niche and phylogenetic position within the genus. We also identified a number of candidates from multiple bacteriocin classes which may be promising antimicrobial candidates when investigated in vitro in future studies.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine (National Development Plan, through the Food Institutional Research Measure, administered by the Department of Agriculture, Fisheries and Food, Ireland 13/F/462)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid2116en
dc.identifier.citationEgan, K., Field, D., Ross, R.P., Cotter, P.D. and Hill, C. (2018) 'In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters within the bacterial genus Geobacillus'. Frontiers in microbiology, 9, 2116. (17pp). doi:10.3389/fmicb.2018.02116en
dc.identifier.doi10.3389/fmicb.2018.02116en
dc.identifier.eissn1664-302X
dc.identifier.endpage17en
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9219
dc.identifier.volume9en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Principal Investigator Programme (PI)/10/IN.1/B3027/IE/Lantibiotics: the next generation/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2018.02116
dc.rights© 2018 Egan, Field, Ross, Cotter and Hill. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriocinen
dc.subjectAntimicrobialen
dc.subjectBioinformaticen
dc.subjectIn silico screeningen
dc.subjectGeobacillusen
dc.titleIn silico prediction and exploration of potential bacteriocin gene clusters within the bacterial genus Geobacillusen
dc.typeArticle (peer-reviewed)en
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