Diversity of natural product biosynthetic genes in the microbiome of the deep sea sponges Inflatella pellicula, Poecillastra compressa and Steletta normani

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dc.contributor.authorJackson, Stephen A.
dc.contributor.authorBorchert, Erik
dc.contributor.authorO'Gara, Fergal
dc.contributor.authorDobson, Alan D. W.
dc.contributor.funderEuropean Commissionen
dc.contributor.funderSeventh Framework Programmeen
dc.contributor.funderMarine Instituteen
dc.date.accessioned2017-02-27T15:19:25Z
dc.date.available2017-02-27T15:19:25Z
dc.date.issued2016-06-29
dc.date.updated2017-02-27T14:59:57Z
dc.description.abstractThree different deep sea sponge species, Inflatella pellicula, Poecillastra compressa, and Stelletta normani comprising seven individual samples, retrieved from depths of 760–2900 m below sea level, were investigated using 454 pyrosequencing for their secondary metabolomic potential targeting adenylation domain and ketosynthase domain sequences. The data obtained suggest a diverse microbial origin of nonribosomal peptide synthetases and polyketide synthase fragments that in part correlates with their respective microbial community structures that were previously described and reveals an untapped source of potential novelty. The sequences, especially the ketosynthase fragments, display extensive clade formations which are clearly distinct from sequences hosted in public databases, therefore highlighting the potential of the microbiome of these deep sea sponges to produce potentially novel small-molecule chemistry. Furthermore, sequence similarities to gene clusters known to be involved in the production of many classes of antibiotics and toxins including lipopeptides, glycopeptides, macrolides, and hepatotoxins were also identified.en
dc.description.sponsorshipMarine Institute (Beaufort Marine Research Award, part of the Sea Change Strategy and the Strategy for Science Technology and Innovation (2006–2012), with the support of The Marine Institute under the Marine Research Sub-Programme of the National Development Plan 2007–2013.)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBorchert, E., Jackson, S. A., O’Gara, F. and Dobson, A. D. W. (2016) 'Diversity of Natural Product Biosynthetic Genes in the Microbiome of the Deep Sea Sponges Inflatella pellicula, Poecillastra compressa, and Stelletta normani', Frontiers in Microbiology, 7(1027). doi:10.3389/fmicb.2016.01027en
dc.identifier.doi10.3389/fmicb.2016.01027
dc.identifier.endpage1027-11en
dc.identifier.issn1664-302X
dc.identifier.journaltitleFrontiers In Microbiologyen
dc.identifier.startpage1027-1en
dc.identifier.urihttps://hdl.handle.net/10468/3699
dc.identifier.volume7en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAINen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEAen
dc.rights© 2016 Borchert, Jackson, O’Gara and Dobson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectMetagenomicsen
dc.subjectPyrosequencingen
dc.subjectDeep sea spongesen
dc.subjectKetosynthase domainen
dc.subjectAdenylation domainen
dc.subjectMicrobiomeen
dc.titleDiversity of natural product biosynthetic genes in the microbiome of the deep sea sponges Inflatella pellicula, Poecillastra compressa and Steletta normanien
dc.typeArticle (peer-reviewed)en
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