Flow injection chemiluminescence determination of isoniazid using luminol and silver nanoparticles
The effect of silver colloidal nanoparticles (AgNPs) on the luminol-isoniazid system was investigated. It was found that AgNPs could act as a nanocatalyst on the luminol-isoniazid system to generate chemiluminescence (CL). The CL emission spectrum of the luminol-isoniazid-AgNPs system showed a peak with a maximum at 425 nm. It was suggested that the luminophor species was the excited state 3-aminophthalate. The reduction of dissolved O2 to H2O2 by isoniazid and decomposition of H2O2 to the oxygen-related radicals were attributed to the catalytic effect of AgNPs. Under optimized conditions, the CL signal intensity was linear with the isoniazid concentration in the range of 10-1000 ng mL- 1, with the correlation coefficient of 0.9996. The limit of detection was 2.7 ng mL- 1 isoniazid. The relative standard deviations for seven repeated measurements of 60 and 200 ng mL- 1 isoniazid were 1.4 and 2.4%, respectively. The effect of potent interfering compounds on the CL signal intensity of the proposed luminol-isoniazid-AgNPs system was investigated. The proposed method was successfully applied to the determination of isoniazid in a pharmaceutical sample.
Chemiluminescence , Flow injection analysis , Isoniazid , Luminol , Silver nanoparticle
Haghighi, B. and Bozorgzadeh, S. (2010) 'Flow injection chemiluminescence determination of isoniazid using luminol and silver nanoparticles', Microchemical Journal, 95 (2), pp. 192-197. doi: 10.1016/j.microc.2009.11.012