Plantaricyclin A, a novel circular bacteriocin produced by Lactobacillus plantarum NI326: purification, characterization, and heterologous production

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dc.contributor.authorBorrero, Juan
dc.contributor.authorKelly, Eoin
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorKelleher, Philip
dc.contributor.authorScully, Colm
dc.contributor.authorCotter, Paul D.
dc.contributor.authorMahony, Jennifer
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderEnterprise Irelanden
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2018-11-22T12:44:42Z
dc.date.available2018-11-22T12:44:42Z
dc.date.issued2017-12-15
dc.date.updated2018-11-22T12:34:50Z
dc.description.abstractBacteriocins from lactic acid bacteria (LAB) are of increasing interest in recent years due to their potential as natural preservatives against food and beverage spoilage microorganisms. In a screening study for LAB, we isolated from olives a strain, Lactobacillus plantarum NI326, with activity against the beverage-spoilage bacterium Alicyclobacillus acidoterrestris. Genome sequencing of NI326 enabled the identification of a gene cluster (designated plc) encoding a putative circular bacteriocin and proteins involved in its modification, transport, and immunity. This novel bacteriocin, named plantaricyclin A (PlcA), was grouped into the circular bacteriocin subgroup II due to its high degree of similarity with other gassericin A-like bacteriocins. Purification of PlcA from the supernatant of Lb. plantarum NI326 resulted in an active peptide with a molecular mass of 5,570 Da, corresponding to that predicted from the (processed) PlcA amino acid sequence. The plc gene cluster was cloned and expressed in Lactococcus lactis NZ9000, resulting in the production of an active 5,570-Da bacteriocin in the supernatant. PlcA is believed to be produced as a 91-amino-acid precursor with a 33-amino-acid leader peptide, which is predicted to be removed, followed by joining of the N and C termini via a covalent linkage to form the mature 58-amino-acid circular bacteriocin PlcA. We report the characterization of a circular bacteriocin produced by Lb. plantarum. The inhibition displayed against A. acidoterrestris highlights its potential use as a preservative in food and beverages.IMPORTANCE In this work, we describe the purification and characterization of an antimicrobial peptide, termed plantaricyclin A (PlcA), produced by a Lactobacillus plantarum strain isolated from olives. This peptide has a circular structure, and all genes involved in its production, circularization, and secretion were identified. PlcA shows antimicrobial activity against different strains, including Alicyclobacillus acidoterrestris, a common spoilage bacterium, which causes substantial economic losses in the beverage industry every year. In this study, we describe a circular antimicrobial peptide, PlcA, for a Lactobacillus plantarum strain.en
dc.description.sponsorshipEnterprise Ireland (Innovation Partnership Programme IP/2013/0254)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide01801-17
dc.identifier.citationBorrero, J., Kelly, E., O'Connor, P. M., Kelleher, P., Scully, C., Cotter, P. D., Mahony, J. and van Sinderen, D. (2017) 'Plantaricyclin A, a novel circular bacteriocin produced by Lactobacillus plantarum NI326: purification, characterization, and heterologous production', Applied and Environmental Microbiology, 84(1), e01801-17 (10pp). doi:10.1128/aem.01801-17en
dc.identifier.doi10.1128/aem.01801-17
dc.identifier.issn0099-2240
dc.identifier.issn1098-5336
dc.identifier.issued1en
dc.identifier.journaltitleApplied and Environmental Microbiologyen
dc.identifier.urihttps://hdl.handle.net/10468/7134
dc.identifier.volume84en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.rights© 2017, American Society for Microbiology. All Rights Reserved.en
dc.subjectCircular bacteriocinen
dc.subjectAlicyclobacillus acidoterrestrisen
dc.subjectLactobacillus plantarumen
dc.subjectImmunityen
dc.titlePlantaricyclin A, a novel circular bacteriocin produced by Lactobacillus plantarum NI326: purification, characterization, and heterologous productionen
dc.typeArticle (peer-reviewed)en
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