Comparative genomics of Lactobacillus crispatus from the gut and vagina reveals genetic diversity and lifestyle adaptation

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dc.contributor.authorZhang, Qiuxiang
dc.contributor.authorZhang, Lili
dc.contributor.authorRoss, R. Paul
dc.contributor.authorZhao, Jianxin
dc.contributor.authorZhang, Hao
dc.contributor.authorChen, Wei
dc.contributor.funderNational Natural Science Foundation of Chinaen
dc.contributor.funderNational First-Class Discipline Program of Food Science and Technologyen
dc.contributor.funderCollaborative Innovationcenter of Food Safety and Quality Control in Jiangsu Provinceen
dc.date.accessioned2023-03-01T10:58:18Z
dc.date.available2023-03-01T10:58:18Z
dc.date.issued2020-03
dc.date.updated2023-03-01T10:44:28Z
dc.description.abstractLactobacillus crispatus colonizes the human feces, human vagina, and the crops and ceca of chicken. To explore the genetic characteristics and evolutionary relationships of L. crispatus isolated from different niches, we selected 37 strains isolated from the human vagina (n = 17), human feces (n = 11), and chicken feces (n = 9), and used comparative genomics to explore the genetic information of L. crispatus from the feces and vagina. No significant difference was found in the three sources of genomic features such as genome size, GC content, and number of protein coding sequences (CDS). However, in a phylogenetic tree constructed based on core genes, vagina-derived L. crispatus and feces-derived strains were each clustered separately. Therefore, the niche exerted an important impact on the evolution of L. crispatus. According to gene annotation, the L. crispatus derived from the vagina possessed a high abundance of genes related to acid tolerance, redox reactions, pullulanase, and carbohydrate-binding modules (CBMs). These genes helped L. crispatus to better adapt to the acidic environment of the vagina and obtain more nutrients, maintaining its dominance in the vagina in competition with other strains. In feces-derived bacteria, more genes encoding CRISPR/Cas system, glycoside hydrolases (GHs) family, and tetracycline/lincomycin resistance genes were found to adapt to the complex intestinal environment. This study highlights the evolutionary relationship of L. crispatus strains isolated from the vagina and feces, and the adaptation of L. crispatus to the host environment.en
dc.description.sponsorshipNational Natural Science Foundation of China (Grant Nos. 31530056 and 31820103010); National First-Class Discipline Program of Food Science and Technology (Grant No.JUFSTR20180102)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid360en
dc.identifier.citationZhang, Q., Zhang, L., Ross, P., Zhao, J., Zhang, H. and Chen, W. (2020) ‘Comparative genomics of Lactobacillus crispatus from the gut and vagina reveals genetic diversity and lifestyle adaptation’, Genes, 11(4), 360 (14pp). doi: 10.3390/genes11040360en
dc.identifier.doi10.3390/genes11040360en
dc.identifier.endpage14en
dc.identifier.issn2073-4425
dc.identifier.issued4en
dc.identifier.journaltitleGenesen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14263
dc.identifier.volume11en
dc.language.isoenen
dc.publisherMDPIen
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAcid toleranceen
dc.subjectComparative genomicsen
dc.subjectEvolutionen
dc.subjectHost adaptationen
dc.subjectLactobacillus crispatusen
dc.titleComparative genomics of Lactobacillus crispatus from the gut and vagina reveals genetic diversity and lifestyle adaptationen
dc.typeArticle (peer-reviewed)en
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