Pregnancy-specific glycoproteins bind integrin alpha IIb beta 3 and inhibit the platelet-fibrinogen interaction

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dc.contributor.authorShanley, Daniel K.
dc.contributor.authorKiely, Patrick A.
dc.contributor.authorGolla, Kalyan
dc.contributor.authorAllen, Seamus
dc.contributor.authorMartin, Kenneth
dc.contributor.authorO'Riordan, Ronan T.
dc.contributor.authorBall, Melanie
dc.contributor.authorAplin, John D.
dc.contributor.authorSinger, Bernhard B.
dc.contributor.authorCaplice, Noel M.
dc.contributor.authorMoran, Niamh
dc.contributor.authorMoore, Thomas F.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHigher Education Authorityen
dc.contributor.funderIrish Governmenten
dc.date.accessioned2016-02-17T11:46:19Z
dc.date.available2016-02-17T11:46:19Z
dc.date.issued2013
dc.description.abstractPregnancy-specific glycoproteins (PSGs) are immunoglobulin superfamily members encoded by multigene families in rodents and primates. In human pregnancy, PSGs are secreted by the syncytiotrophoblast, a fetal tissue, and reach a concentration of up to 400 mu g/ml in the maternal bloodstream at term. Human and mouse PSGs induce release of anti-inflammatory cytokines such as IL-10 and TGF beta 1 from monocytes, macrophages, and other cell types, suggesting an immunoregulatory function. RGD tri-peptide motifs in the majority of human PSGs suggest that they may function like snake venom disintegrins, which bind integrins and inhibit interactions with ligands. We noted that human PSG1 has a KGD, rather than an RGD motif. The presence of a KGD in barbourin, a platelet integrin alpha IIb beta 3 antagonist found in snake venom, suggested that PSG1 may be a selective alpha IIb beta 3 ligand. Here we show that human PSG1 binds alpha IIb beta 3 and inhibits the platelet - fibrinogen interaction. Unexpectedly, however, the KGD is not critical as multiple PSG1 domains independently bind and inhibit alpha IIb beta 3 function. Human PSG9 and mouse Psg23 are also inhibitory suggesting conservation of this function across primate and rodent PSG families. Our results suggest that in species with haemochorial placentation, in which maternal blood is in direct contact with fetal trophoblast, the high expression level of PSGs reflects a requirement to antagonise abundant (3 mg/ml) fibrinogen in the maternal circulation, which may be necessary to prevent platelet aggregation and thrombosis in the prothrombotic maternal environment of pregnancy.en
dc.description.sponsorshipScience Foundation Ireland (SFI Principal Investigator Award); Higher Education Authority (HEA Program for Research in Third Level Institutions 3 (HEA-PRTLI3); Irish Government (Program in Integrative Reproduction funded under the National Development Plan)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide57491
dc.identifier.citationShanley DK, Kiely PA, Golla K, Allen S, Martin K, O’Riordan RT, et al. (2013) Pregnancy-Specific Glycoproteins Bind Integrin αIIbβ3 and Inhibit the Platelet—Fibrinogen Interaction. PLoS ONE 8(2): e57491. doi:10.1371/journal.pone.0057491en
dc.identifier.doi10.1371/journal.pone.0057491
dc.identifier.issn1932-6203
dc.identifier.issued2en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.urihttps://hdl.handle.net/10468/2390
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2013 Shanley et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAlternative activationen
dc.subjectExpressionen
dc.subjectPreeclampsiaen
dc.subjectProteinsen
dc.subjectCoagulationen
dc.subjectSecretionen
dc.subjectMonocytesen
dc.subjectMarkersen
dc.subjectCellsen
dc.subjectPSGen
dc.titlePregnancy-specific glycoproteins bind integrin alpha IIb beta 3 and inhibit the platelet-fibrinogen interactionen
dc.typeArticle (peer-reviewed)en
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