c9, t11, c15-CLNA and c9, t11, t15-CLNA from Lactobacillus plantarum ZS2058 ameliorate DSS-induced colitis in mice

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dc.contributor.authorRen, Qing
dc.contributor.authorYang, Bo
dc.contributor.authorZhang, Hao
dc.contributor.authorRoss, R. Paul
dc.contributor.authorStanton, Catherine
dc.contributor.authorChen, Haiqin
dc.contributor.authorChen, Wei
dc.contributor.funderNational Natural Science Foundation of Chinaen
dc.contributor.funderFundamental Research Funds for the Central Universitiesen
dc.contributor.funderState Key Laboratory of Food Science and Technologyen
dc.date.accessioned2020-03-10T17:02:37Z
dc.date.available2020-03-10T17:02:37Z
dc.date.issued2020-03-03
dc.date.updated2020-03-10T16:50:03Z
dc.description.abstractThe probiotic bacterial strain Lactobacillus plantarum ZS2058 has been proven to alleviate colitis due to its ability to synthesize conjugated fatty acids (CFAs). To investigate the specific functions of CFAs produced by this probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated α-linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and c9, t11, t15-CLNA (CLNA2). The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored. CLNA significantly inhibited weight loss, the disease activity index, colon shortening. Additionally, CLNA improved histological damage, protected colonic mucous layer integrity and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, and claudin-3). CLNA significantly attenuated the expression of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor PPARγ. Moreover, the activity of oxidative stress-related enzymes (superoxide dismutase, glutathione peroxidase and catalase) was increased by CLNA. The myeloperoxidase activity was significantly decreased by CLNA. Meanwhile, the concentrations of CLNA in the liver and colonic conjugated linoleic acid (CLA) were significantly increased in response to CLNA supplementation. Furthermore, CLNA could rebalance the gut microbial composition damaged by DSS, including increasing the α-diversity. CLNA1 and CLNA2 increased the abundance of Ruminococcus and Prevotella, respectively.en
dc.description.sponsorshipNational Natural Science Foundation of China (No. 486 31722041, 31801521, 31530056); Fundamental Research Funds for the Central Universities (No. JUSRP51702A, JUSRP11733); State Key Laboratory of Food Science and Technology (the national first-class discipline program of Food Science and Technology (JUFSTR20180102)); Jiangsu Province “Collaborative Innovation Center for Food Safety and Quality Control”, the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX18_1763)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRen, Q., Yang, B., Zhang, H., Ross, R. P., Stanton, C., Chen, H. and Chen, W. (2020) 'c9, t11, c15-CLNA and c9, t11, t15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate DSS-Induced Colitis in Mice', Journal of Agricultural and Food Chemistry, In Press, doi: 10.1021/acs.jafc.0c00573en
dc.identifier.doi10.1021/acs.jafc.0c00573en
dc.identifier.endpage41en
dc.identifier.issn0021-8561
dc.identifier.journaltitleJournal of Agricultural and Food Chemistryen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9746
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.relation.urihttps://pubs.acs.org/doi/10.1021/acs.jafc.0c00573
dc.rights© 2020 American Chemical Society. This document is the Accepted Manuscript version of a Published Work to appear in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jafc.0c00573en
dc.subjectLactobacillus plantarum ZS2058en
dc.subjectProbioticsen
dc.subjectColitisen
dc.subjectGuten
dc.subjectMicrobialsen
dc.subjectGut microbial compositionen
dc.titlec9, t11, c15-CLNA and c9, t11, t15-CLNA from Lactobacillus plantarum ZS2058 ameliorate DSS-induced colitis in miceen
dc.typeArticle (peer-reviewed)en
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