Development of an antigen-driven colitis model to study presentation of antigens by antigen presenting cells to T cells

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dc.contributor.authorRossini, Valerio
dc.contributor.authorRadulovic, Katarina
dc.contributor.authorRiedel, Christian U.
dc.contributor.authorNiess, Jan Hendrik
dc.contributor.funderSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschungen
dc.date.accessioned2016-11-08T11:57:20Z
dc.date.available2016-11-08T11:57:20Z
dc.date.issued2016-09-18
dc.description.abstractInflammatory bowel disease (IBD) is a chronic inflammation which affects the gastrointestinal tract (GIT). One of the best ways to study the immunological mechanisms involved during the disease is the T cell transfer model of colitis. In this model, immunodeficient mice (RAG-/-recipients) are reconstituted with naive CD4+ T cells from healthy wild type hosts. This model allows examination of the earliest immunological events leading to disease and chronic inflammation, when the gut inflammation perpetuates but does not depend on a defined antigen. To study the potential role of antigen presenting cells (APCs) in the disease process, it is helpful to have an antigen-driven disease model, in which a defined commensal-derived antigen leads to colitis. An antigen driven-colitis model has hence been developed. In this model OT-II CD4+ T cells, that can recognize only specific epitopes in the OVA protein, are transferred into RAG-/- hosts challenged with CFP-OVA-expressing E. coli. This model allows the examination of interactions between APCs and T cells in the lamina propria.en
dc.description.sponsorshipSwiss National Foundation (SNSF 310030_146290)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide54421
dc.identifier.citationRossini, V., Radulovic, K., Riedel, C.U. and Niess, J.H. (2016) ‘Development of an antigen-driven colitis model to study presentation of antigens by antigen presenting cells to T cells’, Journal of Visualized Experiments, (115), e54421 (11pp). doi:10.3791/54421en
dc.identifier.doi10.3791/54421
dc.identifier.endpage11en
dc.identifier.issn1940-087X
dc.identifier.issued115en
dc.identifier.journaltitleJournal of Visualized Experimentsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/3255
dc.language.isoenen
dc.publisherJournal of Visualized Experimentsen
dc.relation.urihttp://www.jove.com/video/54421
dc.rights© 2016, Journal of Visualized Experiments.en
dc.subjectImmunologyen
dc.subjectCX3CR1+ phagocytesen
dc.subjectCD4+ T cellen
dc.subjectColitisen
dc.subjectInflammatory bowel diseaseen
dc.subjectEscherichia colien
dc.subjectConfocal microscopyen
dc.titleDevelopment of an antigen-driven colitis model to study presentation of antigens by antigen presenting cells to T cellsen
dc.typeArticle (peer-reviewed)en
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