Discovery and synthesis of leaderless bacteriocins from the Actinomycetota

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dc.contributor.authorHourigan, Daviden
dc.contributor.authorde Farias, Felipe Micelien
dc.contributor.authorO'Connor, Paula M.en
dc.contributor.authorHill, Colinen
dc.contributor.authorRoss, R. Paulen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Commissionen
dc.contributor.funderHorizon 2020en
dc.date.accessioned2025-09-01T15:04:29Z
dc.date.available2025-09-01T15:04:29Z
dc.date.issued2024-11-24en
dc.description.abstractLeaderless bacteriocins are a unique class of bacteriocins that possess antimicrobial activity after translation and have few cases of documented resistance. Aureocin A53 and lacticin Q are considered two of the most well-studied leaderless bacteriocins. Here, we used in silico genome mining to search for novel aureocin A53-like leaderless bacteriocins in GenBank and MGnify. We identified 757 core peptides across 430 genomes with 75 species found currently without characterized leaderless bacteriocin production. These include putative novel species containing bacteriocin gene clusters (BGCs) from the genera Streptomyces (sp. NBC_00237) and Agrococcus (sp. SL85). To date, all characterized leaderless bacteriocins have been found within the phylum Bacillota, but this study identified 97 core peptides within the phylum Actinomycetota. Members of this phylum are traditionally associated with the production of antibiotics, such is the case with the genus Streptomyces. Actinomycetota is an underexplored phylum in terms of bacteriocin production with no characterized leaderless bacteriocin production to date. The two novel leaderless bacteriocins arcanocin and arachnicin from Actinomycetota members Arcanobacterium sp. and Arachnia sp., respectively, were chemically synthesized and antimicrobial activity was verified. These peptides were encoded in human gut (PRJNA485056) and oral (PRJEB43277) microbiomes, respectively. This research highlights the biosynthetic potential of Actinomycetota in terms of leaderless bacteriocin production and describes the first antimicrobial peptides encoded in the genera Arcanobacterium and Arachnia.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide00298-24en
dc.identifier.citationHourigan, D., Miceli De Farias, F., O'Connor, P.M., Hill, C. and Ross, R.P. (2024) 'Discovery and synthesis of leaderless bacteriocins from the Actinomycetota', Journal of Bacteriology, 206(11), e00298-24 (15pp). https://doi.org/10.1128/jb.00298-24en
dc.identifier.doi10.1128/jb.00298-24en
dc.identifier.eissn1098-5530en
dc.identifier.endpage15en
dc.identifier.issn0021-9193en
dc.identifier.issued11en
dc.identifier.journaltitleJournal of Bacteriologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/17821
dc.identifier.volume206en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/Research Centres Programme::Phase 2/12/RC/2273_P2/IE/APC_Phase 2/en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/HE::ERC::HORIZON-ERC/101054719/EU/Bacteriocins from interbacterial warfare as antibiotic alternative/BACtheWINNERen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-COFUND-FP/101034270/EU/INnovative Sustainable Development InterdisciPlinary Post-Doctoral Research Excellence/INSPIREen
dc.rights© 2024, Hourigan et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriocinsen
dc.subjectActinomycetotaen
dc.subjectLeaderless bacteriocinen
dc.subjectAureocin A53en
dc.subjectAntimicrobial-peptideen
dc.titleDiscovery and synthesis of leaderless bacteriocins from the Actinomycetotaen
dc.typeArticle (peer-reviewed)en
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