Targeting the microbiota to address diet-induced obesity: a time dependent challenge

dc.contributor.authorClarke, Siobhan F.
dc.contributor.authorMurphy, Eileen F.
dc.contributor.authorO'Sullivan, Orla
dc.contributor.authorRoss, R. Paul
dc.contributor.authorO'Toole, Paul W.
dc.contributor.authorShanahan, Fergus
dc.contributor.authorCotter, Paul D.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderTeagascen
dc.contributor.funderAlimentary Healthen
dc.date.accessioned2016-02-17T11:45:33Z
dc.date.available2016-02-17T11:45:33Z
dc.date.issued2013
dc.description.abstractLinks between the gut microbiota and host metabolism have provided new perspectives on obesity. We previously showed that the link between the microbiota and fat deposition is age-and time-dependent subject to microbial adaptation to diet over time. We also demonstrated reduced weight gain in diet-induced obese (DIO) mice through manipulation of the gut microbiota with vancomycin or with the bacteriocin-producing probiotic Lactobacillus salivarius UCC118 (Bac(+)), with metabolic improvement achieved in DIO mice in receipt of vancomycin. However, two phases of weight gain were observed with effects most marked early in the intervention phase. Here, we compare the gut microbial populations at the early relative to the late stages of intervention using a high throughput sequencing-based analysis to understand the temporal relationship between the gut microbiota and obesity. This reveals several differences in microbiota composition over the intervening period. Vancomycin dramatically altered the gut microbiota composition, relative to controls, at the early stages of intervention after which time some recovery was evident. It was also revealed that Bac(+) treatment initially resulted in the presence of significantly higher proportions of Peptococcaceae and significantly lower proportions of Rikenellaceae and Porphyromonadaceae relative to the gut microbiota of L. salivarius UCC118 bacteriocin negative (Bac(-)) administered controls. These differences were no longer evident at the later time. The results highlight the resilience of the gut microbiota and suggest that interventions may need to be monitored and continually adjusted to ensure sustained modification of the gut microbiota.en
dc.description.sponsorshipScience Foundation Ireland (SFI Research Centre grant to the Alimentary Pharmabiotic Centre, Principal Investigator (PI) awards); Teagasc; Alimentary Health Ltd.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide65790
dc.identifier.citationClarke SF, Murphy EF, O’Sullivan O, Ross RP, O’Toole PW, Shanahan F, et al. (2013) Targeting the Microbiota to Address Diet-Induced Obesity: A Time Dependent Challenge. PLoS ONE 8(6): e65790. doi:10.1371/journal.pone.0065790en
dc.identifier.doi10.1371/journal.pone.0065790
dc.identifier.issn1932-6203
dc.identifier.issued6en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.urihttps://hdl.handle.net/10468/2375
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2013 Clarke et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectHuman gut microbiotaen
dc.subjectIntestinal microbiotaen
dc.subjectAntibiotic treatmenten
dc.subjectGenerationen
dc.subjectResistanceen
dc.subjectDiversityen
dc.subjectCapacityen
dc.subjectEcologyen
dc.subjectImpacten
dc.subjectTreesen
dc.titleTargeting the microbiota to address diet-induced obesity: a time dependent challengeen
dc.typeArticle (peer-reviewed)en
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