dc.contributor.author	Clarke, Siobhan F.
dc.contributor.author	Murphy, Eileen F.
dc.contributor.author	O'Sullivan, Orla
dc.contributor.author	Ross, R. Paul
dc.contributor.author	O'Toole, Paul W.
dc.contributor.author	Shanahan, Fergus
dc.contributor.author	Cotter, Paul D.
dc.contributor.funder	Science Foundation Ireland	en
dc.contributor.funder	Teagasc	en
dc.contributor.funder	Alimentary Health	en
dc.date.accessioned	2016-02-17T11:45:33Z
dc.date.available	2016-02-17T11:45:33Z
dc.date.issued	2013
dc.description.abstract	Links between the gut microbiota and host metabolism have provided new perspectives on obesity. We previously showed that the link between the microbiota and fat deposition is age-and time-dependent subject to microbial adaptation to diet over time. We also demonstrated reduced weight gain in diet-induced obese (DIO) mice through manipulation of the gut microbiota with vancomycin or with the bacteriocin-producing probiotic Lactobacillus salivarius UCC118 (Bac(+)), with metabolic improvement achieved in DIO mice in receipt of vancomycin. However, two phases of weight gain were observed with effects most marked early in the intervention phase. Here, we compare the gut microbial populations at the early relative to the late stages of intervention using a high throughput sequencing-based analysis to understand the temporal relationship between the gut microbiota and obesity. This reveals several differences in microbiota composition over the intervening period. Vancomycin dramatically altered the gut microbiota composition, relative to controls, at the early stages of intervention after which time some recovery was evident. It was also revealed that Bac(+) treatment initially resulted in the presence of significantly higher proportions of Peptococcaceae and significantly lower proportions of Rikenellaceae and Porphyromonadaceae relative to the gut microbiota of L. salivarius UCC118 bacteriocin negative (Bac(-)) administered controls. These differences were no longer evident at the later time. The results highlight the resilience of the gut microbiota and suggest that interventions may need to be monitored and continually adjusted to ensure sustained modification of the gut microbiota.	en
dc.description.sponsorship	Science Foundation Ireland (SFI Research Centre grant to the Alimentary Pharmabiotic Centre, Principal Investigator (PI) awards); Teagasc; Alimentary Health Ltd.	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e65790
dc.identifier.citation	Clarke SF, Murphy EF, O’Sullivan O, Ross RP, O’Toole PW, Shanahan F, et al. (2013) Targeting the Microbiota to Address Diet-Induced Obesity: A Time Dependent Challenge. PLoS ONE 8(6): e65790. doi:10.1371/journal.pone.0065790	en
dc.identifier.doi	10.1371/journal.pone.0065790
dc.identifier.issn	1932-6203
dc.identifier.issued	6	en
dc.identifier.journaltitle	PLOS ONE	en
dc.identifier.uri	https://hdl.handle.net/10468/2375
dc.identifier.volume	8	en
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.rights	© 2013 Clarke et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	Human gut microbiota	en
dc.subject	Intestinal microbiota	en
dc.subject	Antibiotic treatment	en
dc.subject	Generation	en
dc.subject	Resistance	en
dc.subject	Diversity	en
dc.subject	Capacity	en
dc.subject	Ecology	en
dc.subject	Impact	en
dc.subject	Trees	en
dc.title	Targeting the microbiota to address diet-induced obesity: a time dependent challenge	en
dc.type	Article (peer-reviewed)	en

Targeting the microbiota to address diet-induced obesity: a time dependent challenge

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