Impact of nisin on Clostridioides difficile and microbiota composition in a faecal fermentation model of the human colon

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dc.contributor.authorO'Reilly, Catherine
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorO'Sullivan, Orla
dc.contributor.authorRea, Mary C.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.date.accessioned2023-03-07T10:11:25Z
dc.date.available2023-03-07T10:11:25Z
dc.date.issued2021-08
dc.date.updated2023-03-06T16:43:45Z
dc.description.abstractAims: Nisin is a bacteriocin with a broad spectrum of activity against Gram-positive bacteria. The aims were to assess nisin activity against Clostridioides difficile in a complex microbial environment and determine the minimum inhibitory concentration at which C. difficile growth is suppressed whilst having minimal impact on the faecal microbiota. Methods and Results: Faecal slurries were prepared from fresh faecal samples and spiked with C. difficile (10(6) CFU per ml). Nisin was added to each fermentation at a range of concentrations from 0 to 500 mu M. Following 24 h, 16S rRNA gene sequencing was performed, and the presence of viable C. difficile was assessed. There was no viable C. difficile detected in the presence of 50-500 mu M nisin. There was a decrease in the diversity of the microbiota in a nisin dose-dependent manner. Nisin predominantly depleted the relative abundance of the Gram-positive bacteria whilst the relative abundance of Gram-negative bacteria such as Escherichia Shigella and Bacteroides increased. Conclusions: Using an ex vivo model of the colon, this study demonstrates the ability of purified nisin to selectively deplete C. difficile in a faecal microbial environment and establishes the minimum concentration at which this occurs whilst having a minimal impact on the composition of the microbiota. Significance and Impact of the Study: This study opens up the potential to use nisin as a therapeutic for clostridial gut infections.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationO’Reilly, C., O’Connor, P.M., O’Sullivan, Ó., Rea, M.C., Hill, C. and Ross, R.P. (2022) ‘Impact of nisin on Clostridioides difficile and microbiota composition in a faecal fermentation model of the human colon’, Journal of Applied Microbiology, 132(2), pp. 1397–1408. doi: 10.1111/jam.15250en
dc.identifier.doi10.1111/jam.15250en
dc.identifier.endpage1408en
dc.identifier.issn1364-5072
dc.identifier.issued2en
dc.identifier.journaltitleJournal of Applied Microbiologyen
dc.identifier.startpage1397en
dc.identifier.urihttps://hdl.handle.net/10468/14286
dc.identifier.volume132en
dc.language.isoenen
dc.publisherJohn Wiley and Sons Inc.en
dc.rights© 2021 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology. This is an open access article under the terms of the Creat ive Commo ns Attri bution-NonCo mmercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposesen
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectIntestinal microbiotaen
dc.subjectNarrow-spectrumen
dc.subjectBacteriocinen
dc.subjectRisken
dc.subjectAntibioticsen
dc.subjectTherapiesen
dc.subjectDiversityen
dc.subjectInfectionen
dc.subjectPregnancyen
dc.subjectDiarrheaen
dc.titleImpact of nisin on Clostridioides difficile and microbiota composition in a faecal fermentation model of the human colonen
dc.typeArticle (peer-reviewed)en
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