dc.contributor.author	Neville, B. Anne
dc.contributor.author	Sheridan, Paul O.
dc.contributor.author	Harris, Hugh Michael B.
dc.contributor.author	Coughlan, Simone
dc.contributor.author	Flint, Harry J.
dc.contributor.author	Duncan, Sylvia H.
dc.contributor.author	Jeffery, Ian B.
dc.contributor.author	Claesson, Marcus J.
dc.contributor.author	Ross, R. Paul
dc.contributor.author	Scott, Karen P.
dc.contributor.author	O'Toole, Paul W.
dc.contributor.funder	Science Foundation Ireland	en
dc.contributor.funder	Irish Research Council for Science Engineering and Technology	en
dc.contributor.funder	Health Research Board	en
dc.contributor.funder	Department of Agriculture, Food and the Marine, Ireland	en
dc.contributor.funder	University College Cork	en
dc.date.accessioned	2016-02-17T11:45:33Z
dc.date.available	2016-02-17T11:45:33Z
dc.date.issued	2013
dc.description.abstract	Some Eubacterium and Roseburia species are among the most prevalent motile bacteria present in the intestinal microbiota of healthy adults. These flagellate species contribute "cell motility" category genes to the intestinal microbiome and flagellin proteins to the intestinal proteome. We reviewed and revised the annotation of motility genes in the genomes of six Eubacterium and Roseburia species that occur in the human intestinal microbiota and examined their respective locus organization by comparative genomics. Motility gene order was generally conserved across these loci. Five of these species harbored multiple genes for predicted flagellins. Flagellin proteins were isolated from R. inulinivorans strain A2-194 and from E. rectale strains A1-86 and M104/1. The amino-termini sequences of the R. inulinivorans and E. rectale A1-86 proteins were almost identical. These protein preparations stimulated secretion of interleukin-8 (IL-8) from human intestinal epithelial cell lines, suggesting that these flagellins were pro-inflammatory. Flagellins from the other four species were predicted to be pro-inflammatory on the basis of alignment to the consensus sequence of pro-inflammatory flagellins from the beta- and gamma-proteobacteria. Many fliC genes were deduced to be under the control of sigma(28). The relative abundance of the target Eubacterium and Roseburia species varied across shotgun metagenomes from 27 elderly individuals. Genes involved in the flagellum biogenesis pathways of these species were variably abundant in these metagenomes, suggesting that the current depth of coverage used for metagenomic sequencing (3.13-4.79 Gb total sequence in our study) insufficiently captures the functional diversity of genomes present at low (<= 1%) relative abundance. E. rectale and R. inulinivorans thus appear to synthesize complex flagella composed of flagellin proteins that stimulate IL-8 production. A greater depth of sequencing, improved evenness of sequencing and improved metagenome assembly from short reads will be required to facilitate in silico analyses of complete complex biochemical pathways for low-abundance target species from shotgun metagenomes.	en
dc.description.sponsorship	Science Foundation Ireland (SFI Principal Investigator Award (07/IN.1/B1780), SFI Award to Alimentary Pharmabiotic Centre (APC)); Irish Research Council for Science Engineering and Technology (Embark studentship); Government of Ireland National Development Plan, Department of Agriculture Food and Marine, Health Research Board (FHRI award to the ELDERMET project); Scottish Government Rural and Environment Science and Analytical Service Division (RESAS), APC University College Cork (Studentship)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e68919
dc.identifier.citation	Neville BA, Sheridan PO, Harris HMB, Coughlan S, Flint HJ, Duncan SH, et al. (2013) Pro-Inflammatory Flagellin Proteins of Prevalent Motile Commensal Bacteria Are Variably Abundant in the Intestinal Microbiome of Elderly Humans. PLoS ONE 8(7): e68919. doi:10.1371/journal.pone.0068919	en
dc.identifier.doi	10.1371/journal.pone.0068919
dc.identifier.issn	1932-6203
dc.identifier.issued	7	en
dc.identifier.journaltitle	PLOS ONE	en
dc.identifier.uri	https://hdl.handle.net/10468/2370
dc.identifier.volume	8	en
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.rights	© 2013 Neville et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	Butyrate producing bacteria	en
dc.subject	Toll-like receptors	en
dc.subject	Human gut	en
dc.subject	Bacillus subtilis	en
dc.subject	Gene expression	en
dc.subject	Escherichia coli	en
dc.subject	Translation initiation	en
dc.subject	Human feces	en
dc.subject	Sequence	en
dc.title	Pro-inflammatory flagellin proteins of prevalent motile commensal bacteria are variably abundant in the intestinal microbiome of elderly humans	en
dc.type	Article (peer-reviewed)	en

Pro-inflammatory flagellin proteins of prevalent motile commensal bacteria are variably abundant in the intestinal microbiome of elderly humans

Files

Original bundle

Collections