Genome mining coupled with OSMAC-based cultivation reveal differential production of surugamide A by the marine sponge isolate Streptomyces sp. SM17 when compared to its terrestrial relative S. albidoflavus J1074

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dc.contributor.authorAlmeida, Eduardo L.
dc.contributor.authorKaur, Navdeep
dc.contributor.authorJennings, Laurence K.
dc.contributor.authorCarrillo Rincón, Andrés Felipe
dc.contributor.authorJackson, Stephen A.
dc.contributor.authorThomas, Olivier P.
dc.contributor.authorDobson, Alan D. W.
dc.contributor.funderConselho Nacional de Desenvolvimento Científico e Tecnológicoen
dc.contributor.funderMarine Instituteen
dc.contributor.funderJiangsu Key Laboratory of Marine Biotechnologyen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-10-23T04:09:35Z
dc.date.available2019-10-23T04:09:35Z
dc.date.issued2019-09-26
dc.description.abstractMuch recent interest has arisen in investigating Streptomyces isolates derived from the marine environment in the search for new bioactive compounds, particularly those found in association with marine invertebrates, such as sponges. Among these new compounds recently identified from marine Streptomyces isolates are the octapeptidic surugamides, which have been shown to possess anticancer and antifungal activities. By employing genome mining followed by an one strain many compounds (OSMAC)-based approach, we have identified the previously unreported capability of a marine sponge-derived isolate, namely Streptomyces sp. SM17, to produce surugamide A. Phylogenomics analyses provided novel insights on the distribution and conservation of the surugamides biosynthetic gene cluster (sur BGC) and suggested a closer relatedness between marine-derived sur BGCs than their terrestrially derived counterparts. Subsequent analysis showed differential production of surugamide A when comparing the closely related marine and terrestrial isolates, namely Streptomyces sp. SM17 and Streptomyces albidoflavus J1074. SM17 produced higher levels of surugamide A than S. albidoflavus J1074 under all conditions tested, and in particular producing >13-fold higher levels when grown in YD and 3-fold higher levels in SYP-NaCl medium. In addition, surugamide A production was repressed in TSB and YD medium, suggesting that carbon catabolite repression (CCR) may influence the production of surugamides in these strains.en
dc.description.sponsorshipMarine Institute through (NMBLI project, Grant-Aid Agreement PBA/MB/16/01); Marine Biotechnology ERA/NET (NEPTUNA project, contract No. PBA/MB/15/02); Science Foundation Ireland (SSPC-2)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid394en
dc.identifier.citationAlmeida, E. L., Kaur, N., Jennings, L. K., Carrillo Rincón, A. F., Jackson, S. A., Thomas, O. P. and Dobson, A. D. W. (2019) 'Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate Streptomyces sp. SM17 When Compared to Its Terrestrial Relative S. albidoflavus J1074', Microorganisms, 7(10), 394. (19pp.) DOI: 10.3390/microorganisms7100394en
dc.identifier.doi10.3390/microorganisms7100394en
dc.identifier.eissn2076-2607
dc.identifier.endpage19en
dc.identifier.issued10en
dc.identifier.journaltitleMicroorganismsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8824
dc.identifier.volume7en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2275/IE/Synthesis and Solid State Pharmaceutical Centre (SSPC)/en
dc.relation.urihttps://www.mdpi.com/2076-2607/7/10/394/htm
dc.rights©2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectGenome miningen
dc.subjectPhylogenomicsen
dc.subjectSecondary metabolitesen
dc.subjectSurugamidesen
dc.subjectSurugamide Aen
dc.subjectMarine sponge-associated bacteriaen
dc.subjectStreptomycesen
dc.subjectAlbidoflavus phylogroupen
dc.titleGenome mining coupled with OSMAC-based cultivation reveal differential production of surugamide A by the marine sponge isolate Streptomyces sp. SM17 when compared to its terrestrial relative S. albidoflavus J1074en
dc.typeArticle (peer-reviewed)en
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