Antibiotic treatment-induced dysbiosis differently affects BDNF and TrkB expression in the brain and in the gut of juvenile mice

Simple item page
dc.contributor.authorBistoletti, Michela
dc.contributor.authorCaputi, Valentina
dc.contributor.authorBaranzini, Nicolò
dc.contributor.authorMarchesi, Nicoletta
dc.contributor.authorFilpa, Viviana
dc.contributor.authorMarsilio, Ilaria
dc.contributor.authorCerantola, Silvia
dc.contributor.authorTerova, Genciana
dc.contributor.authorBaj, Andreina
dc.contributor.authorGrimaldi, Annalisa
dc.contributor.authorPascale, Alessia
dc.contributor.authorFrigo, Gianmario
dc.contributor.authorCrema, Francesca
dc.contributor.authorGiron, Maria Cecilia
dc.contributor.authorGiaroni, Cristina
dc.contributor.funderUniversitĂ  degli Studi dell'Insubriaen
dc.contributor.funderUniversitĂ  degli Studi di Paviaen
dc.contributor.funderUniversitĂ  degli Studi di Padovaen
dc.date.accessioned2019-11-23T06:34:07Z
dc.date.available2019-11-23T06:34:07Z
dc.date.issued2019-02-22
dc.description.abstractAntibiotic use during adolescence may result in dysbiosis-induced neuronal vulnerability both in the enteric nervous system (ENS) and central nervous system (CNS) contributing to the onset of chronic gastrointestinal disorders, such as irritable bowel syndrome (IBS), showing significant psychiatric comorbidity. Intestinal microbiota alterations during adolescence influence the expression of molecular factors involved in neuronal development in both the ENS and CNS. In this study, we have evaluated the expression of brain-derived neurotrophic factor (BDNF) and its high-affinity receptor tropomyosin-related kinase B (TrkB) in juvenile mice ENS and CNS, after a 2-week antibiotic (ABX) treatment. In both mucosa and mucosa-deprived whole-wall small intestine segments of ABX-treated animals, BDNF and TrKB mRNA and protein levels significantly increased. In longitudinal muscle-myenteric plexus preparations of ABX-treated mice the percentage of myenteric neurons staining for BDNF and TrkB was significantly higher than in controls. After ABX treatment, a consistent population of BDNF- and TrkB-immunoreactive neurons costained with SP and CGRP, suggesting up-regulation of BDNF signaling in both motor and sensory myenteric neurons. BDNF and TrkB protein levels were downregulated in the hippocampus and remained unchanged in the prefrontal cortex of ABX-treated animals. Immunostaining for BDNF and TrkB decreased in the hippocampus CA3 and dentate gyrus subregions, respectively, and remained unchanged in the prefrontal cortex. These data suggest that dysbiosis differentially influences the expression of BDNF-TrkB in the juvenile mice ENS and CNS. Such changes may potentially contribute later to the development of functional gut disorders, such as IBS, showing psychiatric comorbidity.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0212856en
dc.identifier.citationBistoletti, M., Caputi, V., Baranzini, N., Marchesi, N., Filpa, V., Marsilio, I., Cerantola, S., Terova, G., Baj, A., Grimaldi, A. and Pascale, A., 2019. Antibiotic treatment-induced dysbiosis differently affects BDNF and TrkB expression in the brain and in the gut of juvenile mice. PloS one, 14(2), (e0212856). DOI:https://doi.org/10.1371/journal.pone.0212856en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0212856en
dc.identifier.eissn1932-6203
dc.identifier.endpage20en
dc.identifier.issued2en
dc.identifier.journaltitlePLoS ONEen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9188
dc.identifier.volume14en
dc.language.isoenen
dc.publisherPLoSen
dc.relation.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212856
dc.rights© 2019 Bistoletti et alen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectTrkB expressionen
dc.subjectEnteric nervous system (ENS)en
dc.subjectCentral nervous system (CNS)en
dc.subjectIrritable bowel syndrome (IBS)en
dc.subjectAntibioticen
dc.titleAntibiotic treatment-induced dysbiosis differently affects BDNF and TrkB expression in the brain and in the gut of juvenile miceen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
journal.pone.0212856.pdf
Size:
2.13 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
APC Microbiome Ireland - Journal Articles