dc.contributor.author	Samson, Julia
dc.contributor.author	Derlipanska, Magdalina
dc.contributor.author	Zaheed, Oza
dc.contributor.author	Dean, Kellie
dc.contributor.funder	University College Cork	en
dc.contributor.funder	Boehringer Ingelheim	en
dc.contributor.funder	Boehringer Ingelheim Fonds	en
dc.contributor.funder	Deutsche Forschungsgemeinschaft	en
dc.contributor.funder	Bundesministerium für Bildung und Forschung	en
dc.date.accessioned	2022-11-03T16:16:41Z
dc.date.available	2022-11-03T16:16:41Z
dc.date.issued	2021-07-08
dc.date.updated	2022-10-25T17:38:15Z
dc.description.abstract	Background: Currently it is unclear how in situ breast cancer progresses to invasive disease; therefore, a better understanding of the events that occur during the transition to invasive carcinoma is warranted. Here we have conducted a detailed molecular and cellular characterization of two, patient-derived, ductal carcinoma in situ (DCIS) cell lines, ETCC-006 and ETCC-010. Methods: Human DCIS cell lines, ETCC-006 and ETCC-010, were compared against a panel of cell lines including the immortalized, breast epithelial cell line, MCF10A, breast cancer cell lines, MCF7 and MDA-MB-231, and another DCIS line, MCF10DCIS.com. Cell morphology, hormone and HER2/ERBB2 receptor status, cell proliferation, survival, migration, anchorage-independent growth, indicators of EMT, cell signalling pathways and cell cycle proteins were examined using immunostaining, immunoblots, and quantitative, reverse transcriptase PCR (qRT-PCR), along with clonogenic, wound-closure and soft agar assays. RNA sequencing (RNAseq) was used to provide a transcriptomic profile. Results: ETCC-006 and ETCC-010 cells displayed notable differences to another DCIS cell line, MCF10DCIS.com, in terms of morphology, steroid-receptor/HER status and markers of EMT. The ETCC cell lines lack ER/PR and HER, form colonies in clonogenic assays, have migratory capacity and are capable of anchorage-independent growth. Despite being isogenic, less than 30% of differentially expressed transcripts overlapped between the two lines, with enrichment in pathways involving receptor tyrosine kinases and DNA replication/cell cycle programs and in gene sets responsible for extracellular matrix organisation and ion transport. Conclusions: For the first time, we provide a molecular and cellular characterization of two, patient-derived DCIS cell lines, ETCC-006 and ETCC-010, facilitating future investigations into the molecular basis of DCIS to invasive ductal carcinoma transition.	en
dc.description.sponsorship	University College Cork (Translational Research Access Programme (TRAP) Award from the School of Medicine); Boehringer Ingelheim (Boehringer Ingelheim Fonds Travel Grant from the Boehringer Ingelheim); Deutsche Forschungsgemeinschaft (Collaborative Research Centre 992 Medical Epigenetics (DFG grant SFB 992/1 2012)); Bundesministerium für Bildung und Forschung (German Federal Ministry of Education and Research (BMBF grants 031 A538A/A538C RBC, 031L0101B/031L0101C de. NBI-epi, 031 L0106 de. STAIR (de. NBI)))	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	790	en
dc.identifier.citation	Samson, J., Derlipanska, M., Zaheed, O. and Dean, K. (2021) ‘Molecular and cellular characterization of two patient-derived ductal carcinoma in situ (Dcis) cell lines, ETCC-006 and ETCC-010’, BMC Cancer, 21(1), 790 (20 pp). https://doi.org/10.1186/s12885-021-08511-2	en
dc.identifier.doi	10.1186/s12885-021-08511-2	en
dc.identifier.endpage	20	en
dc.identifier.issn	1471-2407
dc.identifier.journaltitle	BMC Cancer	en
dc.identifier.startpage	1	en
dc.identifier.uri	https://hdl.handle.net/10468/13813
dc.identifier.volume	21	en
dc.language.iso	en	en
dc.publisher	BMC, part of Springer Nature	en
dc.relation.uri	https://doi.org/10.1186/s12885-021-08511-2
dc.rights	© The Author(s). 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material…	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	Ductal carcinoma in situ (DCIS)	en
dc.subject	Breast carcinoma cell lines	en
dc.subject	qRT-PCR	en
dc.subject	RNA sequencing (RNAseq)	en
dc.subject	Proliferation	en
dc.subject	Migration	en
dc.subject	Anchorage-independent growth	en
dc.subject	Epithelial to mesenchymal transition	en
dc.subject	Cell signalling pathways	en
dc.subject	Cell cycle	en
dc.subject	Breast cancer	en
dc.subject	Beta-catenin	en
dc.subject	Growth	en
dc.subject	Tumors	en
dc.subject	Tumorigenesis	en
dc.subject	Progression	en
dc.title	Molecular and cellular characterization of two patient-derived ductal carcinoma in situ (DCIS) cell lines, ETCC-006 and ETCC-010	en
dc.type	Article (peer-reviewed)	en

Molecular and cellular characterization of two patient-derived ductal carcinoma in situ (DCIS) cell lines, ETCC-006 and ETCC-010

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