Bifidobacterial β-galactosidase-mediated production of galacto-oligosaccharides: structural and preliminary functional assessments

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dc.contributor.authorAmbrogi, Valentinaen
dc.contributor.authorBottacini, Francescaen
dc.contributor.authorMacSharry, Johnen
dc.contributor.authorvan Breen, Justinen
dc.contributor.authorO’Keeffe, Ellenen
dc.contributor.authorWalsh, Danen
dc.contributor.authorSchoemaker, Barryen
dc.contributor.authorCao, Linqiuen
dc.contributor.authorKuipers, Basen
dc.contributor.authorLindner, Cordulaen
dc.contributor.authorJimeno, Maria Luisaen
dc.contributor.authorDoyagüez, Elisa G.en
dc.contributor.authorHernandez-Hernandez, Oswaldoen
dc.contributor.authorMoreno, F. Javieren
dc.contributor.authorSchoterman, Margrieten
dc.contributor.authorvan Sinderen, Douween
dc.contributor.funderFrieslandCampinaen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderFederation of European Microbiological Societiesen
dc.contributor.funderSpanish Ministry of Economy, Industry and Competitivenessen
dc.contributor.funderHorizon 2020en
dc.date.accessioned2023-11-16T15:59:19Z
dc.date.available2023-11-16T15:59:19Z
dc.date.issued2021en
dc.description.abstractIn the current study the ability of four previously characterized bifidobacterial β-galactosidases (designated here as BgaA, BgaC, BgaD, and BgaE) to produce galacto-oligosaccharides (GOS) was optimized. Of these enzymes, BgaA and BgaE were found to be promising candidates for GOS production (and the corresponding GOS mixtures were called GOS-A and GOS-E, respectively) with a GOS concentration of 19.0 and 40.3% (of the initial lactose), respectively. GOS-A and GOS-E were partially purified and structurally characterized. NMR analysis revealed that the predominant (non-lactose) disaccharide was allo-lactose in both purified GOS preparations. The predominant trisaccharide in GOS-A and GOS-E was shown to be 3′-galactosyllactose, with lower levels of 6′-galactosyllactose and 4′-galactosyllactose. These three oligosaccharides have also been reported to occur in human milk. Purified GOS-A and GOS-E were shown to be able to support bifidobacterial growth similar to a commercially available GOS. In addition, GOS-E and the commercially available GOS were shown to be capable of reducing Escherichia coli adhesion to a C2BBe1 cell line. Both in vitro bifidogenic activity and reduced E. coli adhesion support the prebiotic potential of GOS-E and GOS-A.en
dc.description.sponsorshipFederation of European Microbiological Societies (FEMS Research Grant FEMS-RG-2016-0103); Spanish Ministry of Economy, Industry and Competitiveness (Project AGL2017-84614-C2-1-R)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid750635en
dc.identifier.citationAmbrogi, V., Bottacini, F., Mac Sharry, J., Van Breen, J., O’Keeffe, E., Walsh, D., Schoemaker, B., Cao, L., Kuipers, B., Lindner, C., Jimeno, M.L., Doyagüez, E.G., Hernandez-Hernandez, O., Moreno, F.J., Schoterman, M. and Van Sinderen, D. (2021) ‘Bifidobacterial β-galactosidase-mediated production of galacto-oligosaccharides: structural and preliminary functional assessments’, Frontiers in Microbiology, 12, 750635 (19pp). doi: 10.3389/fmicb.2021.750635en
dc.identifier.doi10.3389/fmicb.2021.750635en
dc.identifier.endpage19en
dc.identifier.issn1664-302Xen
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15241
dc.identifier.volume12en
dc.language.isoenen
dc.relation.ispartofFrontiers in Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-IF-GF/843950/EU/Glycomics of Winery Antimicrobial Yeast/GLYCO-WAYen
dc.rights© 2021 Ambrogi, Bottacini, Mac Sharry, van Breen, O’Keeffe, Walsh, Schoemaker, Cao, Kuipers, Lindner, Jimeno, Doyagüez, Hernandez-Hernandez, Moreno, Schoterman and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBifidobacteriumen
dc.subjectPrebioticsen
dc.subjectGut microbiotaen
dc.subjectMicrobiomeen
dc.subjectBifidogenicen
dc.subjectInfanten
dc.subjectOligosaccharidesen
dc.titleBifidobacterial β-galactosidase-mediated production of galacto-oligosaccharides: structural and preliminary functional assessmentsen
dc.typeArticle (peer-reviewed)en
dc.typejournal-articleen
oaire.citation.volume12en
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