LAB as probiotics and silage inoculants for enteric methane inhibition in ruminants

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dc.contributor.advisorStanton, Catherine
dc.contributor.advisorRoss, R. Paul
dc.contributor.advisorHill, Colin
dc.contributor.authorMbandlwa, Philiswaen
dc.contributor.funderFACCE-ERAGAS
dc.date.accessioned2023-09-13T11:43:32Z
dc.date.available2023-09-13T11:43:32Z
dc.date.issued2022-10-19en
dc.date.submitted2022-10-19
dc.description.abstractThere is a constant need for antimicrobial agents and producers that can be used in animal feed and other applications in the agricultural industry. This need justifies the research on novel antimicrobial alternatives such as bacteriocins. Data showed the agricultural environment as a source of LAB, dominated by Lactobacillus species with bacteriocin-producing abilities. Subsequently, an investigation on using CFS and bacteriocins from LAB to reduce methane production in Methanobrevibacter found that L. plantarum (LP58) was most effective in reducing methane from M. gottschalkii while individual strains of L. lactis affected methane production in Methanobrevibacter differently. Purified bacteriocins nukacin and lacticin 3147 did not influence in vitro methane production. The nutritive and fermentative properties of L. plantarum and L. lactis were detailed as silage co-inoculants and these inoculants enhanced the beneficial microbes in the silage and resulted in silage with significantly higher lactic acid concentration. Finally, an animal feeding trial showed significant methane reduction in the dairy cattle fed LAB-treated silage. Gaining insight into the different ruminotypes can enable more targeted approaches for methane mitigation. An investigation of the role of LAB in modifying the rumen microbiome and metabolome showed that LAB positively impacts the rumen by significantly increasing acetate and butyrate production while significantly decreasing Proteobacteria. Livestock farming is a significant sector in the Irish economy as it creates jobs, enables export, and is an intricate part of Irish culture. Although it will be challenging for Irish agriculture to meet its climate targets, concerted efforts, such as using LAB as enteric methane mitigators, can enable Ireland to reach its target of reducing atleast 10% of its GHG emissions by 2030. If overall methane emissions are lowered, the national dairy herd can be maintained or increased, and jobs can be created while continuing to supply meat and milk to the growing world population. Future research will focus on finding even more potent methane reducing LAB and characterisation and elucidating the mechanism of methane inhibition in vitro using LAB CFS.en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMbandlwa, P. 2022. LAB as probiotics and silage inoculants for enteric methane inhibition in ruminants. PhD Thesis, University College Cork.
dc.identifier.endpage279
dc.identifier.urihttps://hdl.handle.net/10468/14961
dc.languageen
dc.publisherUniversity College Corken
dc.rights© 2022, Philiswa Mbandlwa.
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectLactic acid bacteri
dc.subjectMethane
dc.subjectProbiotics
dc.subjectGastrointestinal tract
dc.subjectBacteriocins
dc.titleLAB as probiotics and silage inoculants for enteric methane inhibition in ruminantsen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD - Doctor of Philosophyen
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