Ruthenibacterium lactatiformans gen. nov., sp.nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

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dc.contributor.authorShkoporov, Andrey N.
dc.contributor.authorChaplin, Andrei V.
dc.contributor.authorShcherbakova, Victoria A.
dc.contributor.authorSuzina, Natalia E.
dc.contributor.authorKafarskaia, Lyudmila I.
dc.contributor.authorBozhenko, Vladimir K.
dc.contributor.authorEfimov, Boris A.
dc.date.accessioned2016-10-28T13:38:31Z
dc.date.available2016-10-28T13:38:31Z
dc.date.issued2016-08-01
dc.description.abstractTwo novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1T and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18 : 1 ω9, C18 : 1 ω9 aldehyde, C16 : 0 and C16 : 1 ω7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4–56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 % similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2 % with Gemmiger formicilis ATCC 27749T and 88.9/88.8 % with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae , for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1T (=DSM 100348T=VKM B-2901T).en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationShkoporov, A. N., Chaplin, A. V., Shcherbakova, V. A., Suzina, N. E., Kafarskaia, L. I., Bozhenko, V. K. and Efimov, B. A. (2016) ‘Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces’, International Journal of Systematic and Evolutionary Microbiology, 66, pp. 3041-3049. doi: 10.1099/ijsem.0.001143en
dc.identifier.doi10.1099/ijsem.0.001143
dc.identifier.endpage3049en
dc.identifier.issn1466-5026
dc.identifier.journaltitleInternational Journal of Systematic and Evolutionary Microbiologyen
dc.identifier.startpage3041en
dc.identifier.urihttps://hdl.handle.net/10468/3226
dc.identifier.volume66en
dc.language.isoenen
dc.publisherMicrobiology Societyen
dc.rights© 2016 IUMS. This is the Accepted Paper version of the following article: Shkoporov et al. Int J Syst Evol Microbiol, 66:3041-3049. The Version of Record is available online at http://dx.doi.org/10.1099/ijsem.0.001143en
dc.subjectHuman gut microbiotaen
dc.subjectRuminococcaceaeen
dc.subjectRuthenibacteriumen
dc.subjectLactatiformansen
dc.titleRuthenibacterium lactatiformans gen. nov., sp.nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faecesen
dc.typeArticle (peer-reviewed)en
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