dc.contributor.author	Ortiz-Merino, Raúl A.
dc.contributor.author	Varela, Javier A.
dc.contributor.author	Coughlan, Aisling Y.
dc.contributor.author	Hoshida, Hisashi
dc.contributor.author	da Silveira, Wendel B.
dc.contributor.author	Wilde, Caroline
dc.contributor.author	Kuijpers, Niels G. A.
dc.contributor.author	Geertman, Jan-Maarten
dc.contributor.author	Wolfe, Kenneth H.
dc.contributor.author	Morrissey, John P.
dc.contributor.funder	Seventh Framework Programme
dc.contributor.funder	Consejo Nacional de Ciencia y Tecnología
dc.contributor.funder	Science Foundation Ireland
dc.contributor.funder	Japan Science and Technology Agency
dc.date.accessioned	2018-05-02T10:16:22Z
dc.date.available	2018-05-02T10:16:22Z
dc.date.issued	2018
dc.description.abstract	Kluyveromyces marxianus is traditionally associated with fermented dairy products, but can also be isolated from diverse non-dairy environments. Because of thermotolerance, rapid growth and other traits, many different strains are being developed for food and industrial applications but there is, as yet, little understanding of the genetic diversity or population genetics of this species. K. marxianus shows a high level of phenotypic variation but the only phenotype that has been clearly linked to a genetic polymorphism is lactose utilisation, which is controlled by variation in the LAC12 gene. The genomes of several strains have been sequenced in recent years and, in this study, we sequenced a further nine strains fromdifferent origins. Analysis of the Single Nucleotide Polymorphisms (SNPs) in 14 strains was carried out to examine genome structure and genetic diversity. SNP diversity in K. marxianus is relatively high, with up to 3% DNA sequence divergence between alleles. It was found that the isolates include haploid, diploid, and triploid strains, as shown by both SNP analysis and flow cytometry. Diploids and triploids contain long genomic tracts showing loss of heterozygosity (LOH). All six isolates from dairy environments were diploid or triploid, whereas 6 out 7 isolates from non-dairy environment were haploid. This also correlated with the presence of functional LAC12 alleles only in dairy haplotypes. The diploids were hybrids between a non-dairy and a dairy haplotype, whereas triploids included three copies of a dairy haplotype.	en
dc.description.sponsorship	Consejo Nacional de Ciencia y Tecnología (fellowship number 440667); Japan Science and Technology Agency (Adaptable and the Advanced Low Carbon Technology R&D Program)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	94
dc.identifier.citation	Ortiz-Merino, R. A., Varela, J. A., Coughlan, A. Y., Hoshida, H., da Silveira, W. B., Wilde, C., Kuijpers, N. G. A., Geertman, J.-M., Wolfe, K. H. and Morrissey, J. P. (2018) 'Ploidy variation in Kluyveromyces marxianus separates dairy and non-dairy isolates', Frontiers in Genetics, 9, 94 (16pp). doi: 10.3389/fgene.2018.00094	en
dc.identifier.doi	10.3389/fgene.2018.00094
dc.identifier.issn	1664-8021
dc.identifier.journaltitle	Frontiers in Genetics	en
dc.identifier.uri	https://hdl.handle.net/10468/5930
dc.identifier.volume	9
dc.language.iso	en	en
dc.publisher	Frontiers Media SA	en
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/606795/EU/Yeast Cell Factories: Training Researchers to Apply Modern Post-Genomic Methods In Yeast Biotechnology/YEASTCELL
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1910/IE/Sexual cycles, genomics, and mating-type switching in non-conventional yeast species/
dc.relation.uri	https://www.frontiersin.org/articles/10.3389/fgene.2018.00094/full
dc.rights	© 2018, Ortiz-Merino, Varela, Coughlan, Hoshida, da Silveira, Wilde, Kuipers, Geertman, Wolfe and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Lactose transport	en
dc.subject	Non-conventional yeast	en
dc.subject	Yeast evolution	en
dc.subject	Industrial yeast	en
dc.subject	Dairy	en
dc.subject	Kluyveromyces	en
dc.subject	Lac12	en
dc.title	Ploidy variation in Kluyveromyces marxianus separates dairy and non-dairy isolates	en
dc.type	Article (peer-reviewed)	en

Ploidy variation in Kluyveromyces marxianus separates dairy and non-dairy isolates

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