Depth-dependent differences in community structure of the human colonic microbiota in health

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dc.contributor.authorLavelle, Aonghus
dc.contributor.authorLennon, Grainne
dc.contributor.authorDocherty, Neil
dc.contributor.authorBalfe, Aine
dc.contributor.authorMulcahy, Hugh E.
dc.contributor.authorDoherty, Glen
dc.contributor.authorO'Donoghue, Diarmuid
dc.contributor.authorHyland, John M.
dc.contributor.authorShanahan, Fergus
dc.contributor.authorSheahan, Kieran
dc.contributor.authorCoffey, J. Calvin
dc.contributor.authorWinter, Desmond C.
dc.contributor.authorO'Connell, P. Ronan
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2016-02-17T11:45:31Z
dc.date.available2016-02-17T11:45:31Z
dc.date.issued2013
dc.description.abstractObjective: The aims of this study were to develop techniques for spatial microbial assessment in humans and to establish colonic luminal and mucosal spatial ecology, encompassing longitudinal and cross-sectional axes. Design: A microbiological protected specimen brush was used in conjunction with a biopsy forceps to sample the colon in nine healthy volunteers undergoing colonoscopy. Terminal Restriction Fragment Length Polymorphism analysis was used to determine the major variables in the spatial organization of the colonic microbiota. Results: Protected Specimen Brush sampling retrieved region-specific, uncontaminated samples that were enriched for bacterial DNA and depleted in human DNA when compared to biopsy samples. Terminal Restriction Fragment Length Polymorphism analysis revealed a segmentation of bacterial communities between the luminal brush and biopsy-associated ecological niches with little variability across the longitudinal axis of the colon and reduced diversity in brush samples. Conclusion: These results support the concept of a microbiota with little longitudinal variability but with some degree of segregation between luminal and mucosal communities.en
dc.description.sponsorshipScience Foundation Ireland (SFI Grant 09/IN.1/B2606)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide78835
dc.identifier.citationLavelle A, Lennon G, Docherty N, Balfe A, Mulcahy HE, Doherty G, et al. (2013) Depth-Dependent Differences in Community Structure of the Human Colonic Microbiota in Health. PLoS ONE 8(11): e78835. doi:10.1371/journal.pone.0078835
dc.identifier.doi10.1371/journal.pone.0078835
dc.identifier.issn1932-6203
dc.identifier.issued11en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.urihttps://hdl.handle.net/10468/2359
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2015 Lavelle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectIntestinal microbiotaen
dc.subjectGut microbiotaen
dc.subjectUlcerative colitisen
dc.subjectDiseaseen
dc.subjectDiversityen
dc.subjectEnterotypesen
dc.subjectStabilityen
dc.subjectTherapyen
dc.subjectTracten
dc.subjectFloraen
dc.titleDepth-dependent differences in community structure of the human colonic microbiota in healthen
dc.typeArticle (peer-reviewed)en
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