Detailed mapping of Bifidobacterium strain transmission from mother to infant via a dual culture-based and metagenomic approach

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dc.contributor.authorFeehily, Conoren
dc.contributor.authorO’Neill, Ian J.en
dc.contributor.authorWalsh, Calum J.en
dc.contributor.authorMoore, Rebecca L.en
dc.contributor.authorKilleen, Sarah Louiseen
dc.contributor.authorGeraghty, Aisling A.en
dc.contributor.authorLawton, Elaine M.en
dc.contributor.authorByrne, David F.en
dc.contributor.authorSanchez-Gallardo, Rocioen
dc.contributor.authorNori, Sai Ravi Chandraen
dc.contributor.authorNielsen, Ida Buschen
dc.contributor.authorWortmann, Estheren
dc.contributor.authorMatthews, Elizabethen
dc.contributor.authorO’Flaherty, Roisinen
dc.contributor.authorRudd, Pauline M.en
dc.contributor.authorGroeger, David S.en
dc.contributor.authorShanahan, Fergusen
dc.contributor.authorSaldova, Radkaen
dc.contributor.authorMcAuliffe, Fionnuala M.en
dc.contributor.authorvan Sinderen, Douween
dc.contributor.authorCotter, Paul D.en
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderIrish Research Councilen
dc.contributor.funderHorizon 2020en
dc.date.accessioned2023-12-06T12:19:07Z
dc.date.available2023-12-06T12:19:07Z
dc.date.issued2023en
dc.description.abstractA significant proportion of the infant gut microbiome is considered to be acquired from the mother during and after birth. Thus begins a lifelong and dynamic relationship with microbes that has an enduring impact on host health. Based on a cohort of 135 mother-infant (F = 72, M = 63) dyads (MicrobeMom: ISRCTN53023014), we investigated the phenomenon of microbial strain transfer, with a particular emphasis on the use of a combined metagenomic-culture-based approach to determine the frequency of strain transfer involving members of the genus Bifidobacterium, including species/strains present at low relative abundance. From the isolation and genome sequencing of over 449 bifidobacterial strains, we validate and augment metagenomics-based evidence to reveal strain transfer in almost 50% of dyads. Factors important in strain transfer include vaginal birth, spontaneous rupture of amniotic membranes, and avoidance of intrapartum antibiotics. Importantly, we reveal that several transfer events are uniquely detected employing either cultivation or metagenomic sequencing, highlighting the requirement for a dual approach to obtain an in-depth insight into this transfer process.en
dc.description.sponsorshipIrish Research Council (Fellowship EPSPD/2016/25)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid3015en
dc.identifier.citationFeehily, C., O’Neill, I.J., Walsh, C.J., Moore, R.L., Killeen, S.L., Geraghty, A.A., Lawton, E.M., Byrne, D., Sanchez-Gallardo, R., Nori, S.R.C., Nielsen, I.B., Wortmann, E., Matthews, E., O’Flaherty, R., Rudd, P.M., Groeger, D., Shanahan, F., Saldova, R., McAuliffe, F.M., Van Sinderen, D. and Cotter, P.D. (2023) ‘Detailed mapping of Bifidobacterium strain transmission from mother to infant via a dual culture-based and metagenomic approach’, Nature Communications, 14(1), 3015 (14pp). doi: 10.1038/s41467-023-38694-0en
dc.identifier.doi10.1038/s41467-023-38694-0en
dc.identifier.endpage14en
dc.identifier.issn2041-1723en
dc.identifier.issued1en
dc.identifier.journaltitleNature Communications,en
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15305
dc.identifier.volume14en
dc.language.isoenen
dc.publisherNature Researchen
dc.relation.ispartofNature Communicationsen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Spokes Programme::Fixed Call/16/SP/3827/IE/Determinants of Mother-to-Infant Transfer of Microbiota/en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-COFUND-FP/754535/EU/APC Postdoctoral EXcellence Programme/APEXen
dc.rights© The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectInfant gut microbiomeen
dc.subjectMother-infanten
dc.subjectBifidobacterial strainsen
dc.subjectMetagenomic sequencingen
dc.subjectMetagenomic approachen
dc.titleDetailed mapping of Bifidobacterium strain transmission from mother to infant via a dual culture-based and metagenomic approachen
dc.typeArticle (peer-reviewed)en
dc.typejournal-articleen
oaire.citation.issue1en
oaire.citation.volume14en
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