Real-time PCR methods for the detection of blown pack spoilage causing Clostridium species; C. estertheticum, C. gasigenes and C. ruminantium

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dc.check.date2018-06-03
dc.check.infoAccess to this article is restricted until 12 months after publication by request of the publisher.en
dc.contributor.authorReid, Rachael
dc.contributor.authorBurgess, Catherine M.
dc.contributor.authorMcCabe, Evonne
dc.contributor.authorFanning, Séamus
dc.contributor.authorWhyte, Paul
dc.contributor.authorKerry, Joseph P.
dc.contributor.authorBolton, Declan
dc.contributor.funderTeagascen
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.date.accessioned2017-09-05T11:08:09Z
dc.date.available2017-09-05T11:08:09Z
dc.date.issued2017-06-03
dc.date.updated2017-09-05T10:55:34Z
dc.description.abstractA set of real-time PCR methods for the detection of C. estertheticum, C. gasigenes and C. ruminantium, the causative agents of blown pack spoilage (BPS) in vacuum packaged beef, was developed. Robust validation of the sensitivity and specificity was carried out in the three matrices (beef meat drip, wet environmental swabs and dry environmental swabs) as encountered in our testing laboratory and against Clostridium strains (n = 76) and non-Clostridium strains (n = 36). It was possible to detect 4–5 spores per ml for C. estertheticum, 2 spores per ml for C. gasigenes and 8 spores per ml for C. ruminantium, without the need for enrichment of the samples. This high sensitivity is particularly important for the beef sector, not just for testing spoiled product but also in the early detection of contaminated beef and in validation of sporicidal cleaning procedures for critical pieces of equipment such as the vacuum packaging machine, which have the potential to contaminate large volumes of product.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine (Food Institutional Research Measure (FIRM) project 11F033)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationReid, R., Burgess, C. M., McCabe, E., Fanning, S., Whyte, P., Kerry, J. and Bolton, D. (2017) 'Real-time PCR methods for the detection of blown pack spoilage causing Clostridium species; C. estertheticum, C. gasigenes and C. ruminantium', Meat Science, 133, pp. 56-60. doi:10.1016/j.meatsci.2017.06.001en
dc.identifier.doi10.1016/j.meatsci.2017.06.001
dc.identifier.endpage60en
dc.identifier.issn0309-1740
dc.identifier.journaltitleMeat Scienceen
dc.identifier.startpage56en
dc.identifier.urihttps://hdl.handle.net/10468/4631
dc.identifier.volume133en
dc.language.isoenen
dc.publisherElsevier Ltden
dc.rights© 2017, Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectReal-time PCRen
dc.subjectBlown pack spoilageen
dc.subjectClostridium estertheticumen
dc.subjectClostridium gasigenesen
dc.subjectClostridium ruminantiumen
dc.titleReal-time PCR methods for the detection of blown pack spoilage causing Clostridium species; C. estertheticum, C. gasigenes and C. ruminantiumen
dc.typeArticle (peer-reviewed)en
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