A cell wall-associated polysaccharide is required for bacteriophage adsorption to the Streptococcus thermophilus cell surface

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dc.contributor.authorMcDonnell, Brian
dc.contributor.authorHanemaaijer, Laurens
dc.contributor.authorBottacini, Francesca
dc.contributor.authorKelleher, Philip
dc.contributor.authorLavelle, Katherine
dc.contributor.authorSadovskaya, Irina
dc.contributor.authorVinogradov, Evgeny
dc.contributor.authorVer Loren van Themaat, Emiel
dc.contributor.authorKouwen, Thijs
dc.contributor.authorMahony, Jennifer
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderUniversité Lille 1 - Sciences et Technologiesen
dc.contributor.funderDSM Food Specialities, The Netherlandsen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2020-03-06T10:56:11Z
dc.date.available2020-03-06T10:56:11Z
dc.date.issued2020
dc.date.updated2020-03-06T10:37:33Z
dc.description.abstractStreptococcus thermophilus strain ST64987 was exposed to a member of a recently discovered group of S. thermophilus phages (the 987 phage group), generating phage-insensitive mutants, which were then characterized phenotypically and genomically. Decreased phage adsorption was observed in selected bacteriophage-insensitive mutants, and was partnered with a sedimenting phenotype and increased cell chain length or aggregation. Whole genome sequencing of several bacteriophage-insensitive mutants identified mutations located in a gene cluster presumed to be responsible for cell wall polysaccharide production in this strain. Analysis of cell surface-associated glycans by methylation and NMR spectroscopy revealed a complex branched rhamno-polysaccharide in both ST64987 and phage-insensitive mutant BIM3. In addition, a second cell wall-associated polysaccharide of ST64987, composed of hexasaccharide branched repeating units containing galactose and glucose, was absent in the cell wall of mutant BIM3. Genetic complementation of three phage-resistant mutants was shown to restore the carbohydrate and phage resistance profiles of the wild-type strain, establishing the role of this gene cluster in cell wall polysaccharide production and phage adsorption and, thus, infection.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMcDonnell, B., Hanemaaijer, L., Bottacini, F., Kelleher, P., Lavelle, K., Sadovskaya, I., Vinogradov, E., Ver Loren van Themaat, E., Kouwen, T., Mahony, J. and van Sinderen, D. 'A cell wall-associated polysaccharide is required for bacteriophage adsorption to the Streptococcus thermophilus cell surface', Molecular Microbiology,en
dc.identifier.doi10.1111/mmi.14494en
dc.identifier.endpage15en
dc.identifier.issn0950-382X
dc.identifier.journaltitleMolecular Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9729
dc.language.isoenen
dc.publisherWileyen
dc.relation.projectUniversité Lille (PAGés platform (Plateforme d’Analyses des Glycoconjugués. UMR8576, Faculté des Sciences, Université de Lille, CNRS, 59000 Lille));en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/en
dc.relation.project17 SP 4678en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.urihttps://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.14494
dc.rights© 2020 John Wiley & Sons Ltd. This is the peer reviewed version of the following article: McDonnell, B, Hanemaaijer, L, Bottacini, F, et al. A cell wall‐associated polysaccharide is required for bacteriophage adsorption to the Streptococcus thermophilus cell surface. Mol Microbiol. 2020, which has been published in final form at https://doi.org/10.1111/mmi.14494. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en
dc.subjectAdsorptionen
dc.subjectDairyen
dc.subjectMutationen
dc.subjectNMRen
dc.subjectPolysaccharideen
dc.subjectStructureen
dc.titleA cell wall-associated polysaccharide is required for bacteriophage adsorption to the Streptococcus thermophilus cell surfaceen
dc.typeArticle (peer-reviewed)en
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