Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels

dc.contributor.authorFang, Fang
dc.contributor.authorLi, Yin
dc.contributor.authorBumann, Mario
dc.contributor.authorRaftis, Emma J.
dc.contributor.authorCasey, Pat G.
dc.contributor.authorCooney, Jakki C.
dc.contributor.authorWalsh, Martin A.
dc.contributor.authorO'Toole, Paul W.
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2010-03-15T11:50:01Z
dc.date.available2010-03-15T11:50:01Z
dc.date.issued2009-09
dc.description.abstractCommensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.en
dc.description.sponsorshipScience Foundation Ireland (Centre for Science, Engineering and Technology).en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationFang F., Li Y., Bumann M., Raftis E.J., Casey P.G., Cooney J.C., Walsh M.A., O'Toole P.W. 2009. Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels. Journal of Bacteriology 191(18):5743-57.en
dc.identifier.doi10.1128/JB.00506-09
dc.identifier.endpage5757en
dc.identifier.issn0021-9193
dc.identifier.issn1098-5530
dc.identifier.issued18en
dc.identifier.journaltitleJournal of Bacteriologyen
dc.identifier.startpage5743en
dc.identifier.urihttps://hdl.handle.net/10468/120
dc.identifier.volume191en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urihttp://jb.asm.org/cgi/content/full/191/18/5743/DC1
dc.rights© American Society for Microbiologyen
dc.subjectBile salt hydrolase enzymeen
dc.subject.lcshLactobacillus -- Geneticsen
dc.subject.lcshBileen
dc.titleAllelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levelsen
dc.typeArticle (peer-reviewed)en
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