A new phage lysin isolated from the oral microbiome targeting streptococcus pneumoniae

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dc.contributor.authorvan der Kamp, Immeen
dc.contributor.authorDraper, Lorraine A.en
dc.contributor.authorSmith, Muireann K.en
dc.contributor.authorButtimer, Colinen
dc.contributor.authorRoss, R. Paulen
dc.contributor.authorHill, Colinen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderIrish Research Councilen
dc.date.accessioned2024-03-28T14:30:41Z
dc.date.available2024-03-28T14:30:41Z
dc.date.issued2020-01-01en
dc.description.abstractStreptococcus pneumoniae is highly pathogenic and causes several mucosal and invasive infections. Due to the rising number of multidrug-resistant (MDR) strains of S. pneumoniae, new antimicrobials with alternative mechanisms of action are urgently needed. In this study, we identified two new Streptococcal phages from the oral microbiome, 23TH and SA01. Their lysins, 23TH_48 and SA01_53, were recombinantly expressed, characterized and tested for their lethality. SA01_53 was found to only lyse its host strain of S. anginosus, while 23TH_48 was found to possess a broader lytic activity beyond its host strain of S. infantis, with several S. pneumoniae isolates sensitive to its lytic activity. 23TH_48 at a concentration of five activity units per mL (U/mL) was found to reduce cell counts of S. pneumoniae DSM 24048 by 4 log10 colony forming units per mL (CFU/mL) within 1 h and effectively prevented and destroyed biofilms of S. pneumoniae R6 at concentrations of 228.8 ng/µL and 14.3 ng/µL, respectively. Given its high lytic activity, 23TH_48 could prove to be a promising candidate to help combat pneumococcal infections.en
dc.description.sponsorshipIrish Research Council (Government of Ireland Postdoctoral Fellowship 2019, Project ID GOIPD/2019/1097))en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid478en
dc.identifier.citationVan Der Kamp, I., Draper, L.A., Smith, M.K., Buttimer, C., Ross, R.P. and Hill, C. (2020) ‘A new phage lysin isolated from the oral microbiome targeting streptococcus pneumoniae’, Pharmaceuticals, 13(12), 478 (19pp). doi: 10.3390/ph13120478en
dc.identifier.doi10.3390/ph13120478en
dc.identifier.eissn1424-8247en
dc.identifier.endpage19en
dc.identifier.issued12en
dc.identifier.journaltitlePharmaceuticalsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15718
dc.identifier.volume13en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectStreptococcus pneumoniaeen
dc.subjectBacteriophageen
dc.subjectLysinsen
dc.subjectAntimicrobial therapyen
dc.subjectBiofilmsen
dc.subject~Microbiology - Journal Articles~en
dc.titleA new phage lysin isolated from the oral microbiome targeting streptococcus pneumoniaeen
dc.typeArticle (peer-reviewed)en
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