Transposon mutagenesis in bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for bifidobacterium breve UCC2003

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dc.contributor.authorRuiz, Lorena
dc.contributor.authorO'Connell Motherway, Mary
dc.contributor.authorLanigan, Noreen
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderHealth Research Boarden
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2016-08-31T14:20:55Z
dc.date.available2016-08-31T14:20:55Z
dc.date.issued2013-05-30
dc.description.abstractBifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years. However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B. breve UCC2003 and B. breve NCFB2258. We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. breve UCC2003 and a Tn5-based transposome strategy. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities.en
dc.description.sponsorshipScience Foundation Ireland (Irish Government’s National Development Plan; SFI grants 02/CE/B124 and 07/CE/B1368); HRB postdoctoral fellowship (grant PDTM/20011/9)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRuiz, L., O’Connell Motherway, M., Lanigan N. and van Sinderen D. (2013) 'Transposon mutagenesis in bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for bifidobacterium breve UCC2003', PLoS ONE 8(5): e64699. http://dx.doi.org/10.1371/journal.pone.0064699en
dc.identifier.doi10.1371/journal.pone.0064699
dc.identifier.endpagee64699-10en
dc.identifier.issn1932-6203
dc.identifier.issued5en
dc.identifier.journaltitlePLoS Oneen
dc.identifier.startpagee64699-1en
dc.identifier.urihttps://hdl.handle.net/10468/3042
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPLoS ONEen
dc.rights© 2013 Ruiz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/en
dc.subjectBifidobacterium breveen
dc.subjectTransposon insertion mutantsen
dc.subjectBifidobacteriaen
dc.subjectGastrointestinal disordersen
dc.subjectMetabolic disordersen
dc.titleTransposon mutagenesis in bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for bifidobacterium breve UCC2003en
dc.typeArticle (peer-reviewed)en
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