Comparative analysis of Lactobacillus gasseri from Chinese subjects reveals a new species-level taxa

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dc.contributor.authorZhou, Xingya
dc.contributor.authorYang, Bo
dc.contributor.authorStanton, Catherine
dc.contributor.authorRoss, R. Paul
dc.contributor.authorZhao, Jianxin
dc.contributor.authorZhang, Hao
dc.contributor.authorChen, Wei
dc.contributor.funderNational Natural Science Foundation of Chinaen
dc.contributor.funderFundamental Research Funds for the Central Universitiesen
dc.contributor.funderNational First-Class Discipline Program of Food Science and Technologyen
dc.contributor.funderCollaborative Innovationcenter of Food Safety and Quality Control in Jiangsu Provinceen
dc.date.accessioned2023-03-10T12:42:52Z
dc.date.available2023-03-10T12:42:52Z
dc.date.issued2020-02
dc.date.updated2023-03-09T11:46:49Z
dc.description.abstractBackground: Lactobacillus gasseri as a probiotic has history of safe consumption is prevalent in infants and adults gut microbiota to maintain gut homeostasis. Results: In this study, to explore the genomic diversity and mine potential probiotic characteristics of L. gasseri, 92 strains of L. gasseri were isolated from Chinese human feces and identified based on 16 s rDNA sequencing, after draft genomes sequencing, further average nucleotide identity (ANI) value and phylogenetic analysis reclassified them as L. paragasseri (n = 79) and L. gasseri (n = 13), respectively. Their pan/core-genomes were determined, revealing that L. paragasseri had an open pan-genome. Comparative analysis was carried out to identify genetic features, and the results indicated that 39 strains of L. paragasseri harboured Type II-A CRISPR-Cas system while 12 strains of L. gasseri contained Type I-E and II-A CRISPR-Cas systems. Bacteriocin operons and the number of carbohydrate-active enzymes were significantly different between the two species. Conclusions: This is the first time to study pan/core-genome of L. gasseri and L. paragasseri, and compare their genetic diversity, and all the results provided better understating on genetics of the two species.en
dc.description.sponsorshipNational Natural Science Foundation of China (Nos. 31530056, 31820103010, 31801521); Fundamental Research Funds for the Central Universities (No. JUSRP11733); National First-Class Discipline Program of Food Science and Technology (JUFSTR20180102)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid119en
dc.identifier.citationZhou, X., Yang, B., Stanton, C., Ross, R.P., Zhao, J., Zhang, H. and Chen, W. (2020) ‘Comparative analysis of Lactobacillus gasseri from Chinese subjects reveals a new species-level taxa’, BMC Genomics, 21(1), 119 (16pp). doi: 10.1186/s12864-020-6527-yen
dc.identifier.doi10.1186/s12864-020-6527-yen
dc.identifier.endpage16en
dc.identifier.issn1471-2164
dc.identifier.issued1en
dc.identifier.journaltitleBMC Genomicsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14301
dc.identifier.volume21en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rights© The Author(s). 2020 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectANIen
dc.subjectGenotypeen
dc.subjectLactobacillus gasserien
dc.subjectLactobacillus paragasserien
dc.subjectPan/core-genomeen
dc.titleComparative analysis of Lactobacillus gasseri from Chinese subjects reveals a new species-level taxaen
dc.typeArticle (peer-reviewed)en
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