Interaction between Candida albicans and Pseudomonas aeruginosa

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dc.check.embargoformatNot applicableen
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dc.contributor.advisorMorrissey, John P.en
dc.contributor.authorKonstantinidou, Nina
dc.contributor.funderHigher Education Authorityen
dc.date.accessioned2016-08-22T11:47:10Z
dc.date.available2016-08-22T11:47:10Z
dc.date.issued2016
dc.date.submitted2016
dc.description.abstractFungal pathogen Candida albicans causes serious nosocomial infections in patients, in part, due to formation of drug-resistant biofilms. Protein kinases (PK) and transcription factors (TF) mediate signal transduction and transcription of proteins involved in biofilm development. To discover biofilm-related PKs, a collection of 63 C. albicans PK mutants was screened twice independently with microtiter plate-based biofilm assay (XTT). Thirty-eight (60%) mutants showed different degrees of biofilm impairment with the poor biofilm formers additionally possessing filamentation defects. Most of these genes were already known to encode proteins associated with Candida morphology and biofilms but VPS15, PKH3, PGA43, IME2 and CEX1, were firstly associated with both processes in this study. Previous studies of Holcombe et al. (2010) had shown that bacterial pathogen, Pseudomonas aeruginosa can impair C. albicans filamentation and biofilm development. To investigate their interaction, the good biofilm former PK mutants of C. albicans were assessed for their response to P. aeruginosa supernatants derived from two strains, wildtype PAO1 and homoserine lactone (HSL)-free mutant ΔQS, without finding any nonresponsive mutants. This suggested that none of the PKs in this study was implicated in Candida-Pseudomonas signaling. To screen promoter sequences for overrepresented TFs across C. albicans gene sets significantly up/downregulated in presence of bacterial supernatants from Holcombe et al. (2010) study, TFbsST database was created online. The TFbsST database integrates experimentally verified TFs of Candida to analyse promoter sequences for TF binding sites. In silico studies predicted that Efg1p was overrepresented in C. albicans and C. parapsilosis RBT family genes.en
dc.description.sponsorshipHigher Education Authority (PRTLI5 Structured PhD programme)en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationKonstantinidou, N. 2016. Interaction between Candida albicans and Pseudomonas aeruginosa. PhD Thesis, University College Cork.en
dc.identifier.endpage150en
dc.identifier.urihttps://hdl.handle.net/10468/3015
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2016, Nina Konstantinidou.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectCandida albicansen
dc.subjectCandida albicans morphology and biofilmsen
dc.subjectPseudomonas aeruginosaen
dc.subjectTFbsST databaseen
dc.subjectIn silico analysis of Candida albicans promoter sequencesen
dc.subjectProtein kinaseen
dc.subjectBiofilmsen
dc.subjectFilamentationen
dc.subjectTranscription factor binding site motifsen
dc.subjectPromoter regionsen
dc.subjectYeast hyphaeen
dc.subjectCandida albicans morphology and interaction with Pseudomonas aeruginosaen
dc.thesis.opt-outfalse
dc.titleInteraction between Candida albicans and Pseudomonas aeruginosaen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoral Degree (Structured)en
dc.type.qualificationnamePhD (Science)en
ucc.workflow.supervisorj.morrissey@ucc.ie
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