Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1

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dc.contributor.authorMahony, Jennifer
dc.contributor.authorAlqarni, Mona
dc.contributor.authorStockdale, Stephen R.
dc.contributor.authorSpinelli, Silvia
dc.contributor.authorFeyereisen, Marine
dc.contributor.authorCambillau, Christian
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderSaudi Arabian Cultural Bureau
dc.date.accessioned2016-12-02T09:43:15Z
dc.date.available2016-12-02T09:43:15Z
dc.date.issued2016-11-08
dc.date.updated2016-12-02T09:28:48Z
dc.description.abstractThe tail tape measure protein (TMP) of tailed bacteriophages (also called phages) dictates the tail length and facilitates DNA transit to the cell cytoplasm during infection. Here, a thorough mutational analysis of the TMP from lactococcal phage TP901-1 (TMPTP901-1) was undertaken. We generated 56 mutants aimed at defining TMPTP901-1 domains that are essential for tail assembly and successful infection. Through analysis of the derived mutants, we determined that TP901-1 infectivity requires the N-terminal 154 aa residues, the C-terminal 60 residues and the first predicted hydrophobic region of TMPTP901-1 as a minimum. Furthermore, the role of TMPTP901-1 in tail length determination was visualized by electron microscopic imaging of TMP-deletion mutants. The inverse linear correlation between the extent of TMPTP901-1-encoding gene deletions and tail length of the corresponding virion provides an estimate of TMPTP901-1 regions interacting with the connector or involved in initiator complex formation. This study represents the most thorough characterisation of a TMP from a Gram-positive host-infecting phage and provides essential advances to understanding its role in virion assembly, morphology and infection.en
dc.description.sponsorshipScience Foundation Ireland (Technology Innovation Development Award (TIDA) Ref. No. 14/TIDA/2287; Principal Investigator Award Ref. No. 13/IA/1953); Saudi Arabian Cultural Bureau (King Abdullah Scholarship)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid36667
dc.identifier.citationMahony, J., Alqarni, M., Stockdale, S., Spinelli, S., Feyereisen, M., Cambillau, C. and van Sinderen, D. (2016) 'Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1', Scientific Reports, 6, pp. 1-10. doi:10.1038/srep36667en
dc.identifier.doi10.1038/srep36667
dc.identifier.endpage10en
dc.identifier.issn2045-2322
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/3342
dc.identifier.volume6en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rights© 2016, the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriophage lambda tailen
dc.subjectLength determinationen
dc.subjectMembrane proteinen
dc.subjectInfectionen
dc.subjectBaseplateen
dc.subjectReceptoren
dc.subjectHosten
dc.subjectChaperoneen
dc.subjectLactisen
dc.subjectGenesen
dc.titleFunctional and structural dissection of the tape measure protein of lactococcal phage TP901-1en
dc.typeArticle (peer-reviewed)en
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