Administration of a live culture of Lactococcus lactis DPC 3147 into the bovine mammary gland stimulates the local host immune response, particularly IL-1beta and IL-8 gene expression

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dc.contributor.authorBeecher, Christine
dc.contributor.authorDaly, Mairead
dc.contributor.authorBerry, Donagh P.
dc.contributor.authorKlostermann, Katja
dc.contributor.authorFlynn, James
dc.contributor.authorMeaney, William
dc.contributor.authorHill, Colin
dc.contributor.authorMcCarthy, Tommie V.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorGiblin, Linda
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderTeagascen
dc.contributor.funderDairy Research Trust, Ireland
dc.date.accessioned2013-04-29T14:58:21Z
dc.date.available2013-04-29T14:58:21Z
dc.date.copyright2009
dc.date.issued2009-02
dc.description.abstractMastitis is one of the most costly diseases to the dairy farming industry. Conventional antibiotic therapy is often unsatisfactory for successful treatment of mastitis and alternative treatments are continually under investigation. We have previously demonstrated, in two separate field trials, that a probiotic culture, Lactococcus lactis DPC 3147, was comparable to antibiotic therapy to treat bovine mastitis. To understand the mode of action of this therapeutic, we looked at the detailed immune response of the host to delivery of this live strain directly into the mammary gland of six healthy dairy cows. All animals elicited signs of udder inflammation 7 h post infusion. At this time, clots were visible in the milk of all animals in the investigation. The most pronounced increase in immune gene expression was observed in Interleukin (IL)-1β and IL-8, with highest expression corresponding to peaks in somatic cell count. Infusion with a live culture of a Lc. lactis leads to a rapid and considerable innate immune response.en
dc.description.sponsorshipScience Foundation Ireland (SFI-CSET); Teagasc (Walsh Fellowship); Teagasc (Teagasc Retooling Programme under the National Development Plan)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBeecher C, Daly M, Berry DP, Klostermann K, Flynn J, Meaney W, Hill C, McCarthy TV, Ross RP, Giblin L. (2009) Administration of a live culture of Lactococcus lactis DPC 3147 into the bovine mammary gland stimulates the local host immune response, particularly IL-1beta and IL-8 gene expression. Journal of Dairy Research, 76(3):340-8. doi: 10.1017/S0022029909004154en
dc.identifier.doi10.1017/S0022029909004154
dc.identifier.endpage348en
dc.identifier.issued3en
dc.identifier.journaltitleJournal of Dairy Researchen
dc.identifier.startpage340en
dc.identifier.urihttps://hdl.handle.net/10468/1099
dc.identifier.volume76en
dc.language.isoenen
dc.publisherCambridge University Pressen
dc.relation.urihttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=6015060&fulltextType=RA&fileId=S0022029909004154
dc.rights© Proprietors of Journal of Dairy Research 2009en
dc.subjectMastitisen
dc.subjectLactococcus lactisen
dc.subjectProbiotic treatmenten
dc.subject.lcshMicrobiology.en
dc.titleAdministration of a live culture of Lactococcus lactis DPC 3147 into the bovine mammary gland stimulates the local host immune response, particularly IL-1beta and IL-8 gene expressionen
dc.typeArticle (peer-reviewed)en
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