Oral delivery of nisin in resistant starch based matrices alters the gut microbiota in mice

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dc.contributor.authorGough, Ronan
dc.contributor.authorRubio, Raul Cabrera
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorCrispie, Fiona
dc.contributor.authorBrodkorb, André
dc.contributor.authorMiao, Song
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.contributor.authorCotter, Paul D.
dc.contributor.authorNilaweera, Kanishka N.
dc.contributor.authorRea, Mary C.
dc.contributor.funderBiotechnology and Biological Sciences Research Council
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderTeagasc
dc.contributor.funderDepartment of Agriculture, Food and the Marine
dc.date.accessioned2018-09-20T15:53:42Z
dc.date.available2018-09-20T15:53:42Z
dc.date.issued2018-01-01
dc.description.abstractThere is a growing recognition of the role the gastrointestinal microbiota plays in health and disease. Ingested antimicrobial proteins and peptides have the potential to alter the gastrointestinal microbiota; particularly if protected from digestion. Nisin is an antimicrobial peptide that is used as a food preservative. This study examined the ability of nisin to affect the murine microbiota when fed to mice in two different starch based matrices; a starch dough comprising raw starch granules and a starch gel comprising starch that was gelatinized and retrograded. The effects of the two starch matrices by themselves on the microbiota were also examined. Following 16S rRNA compositional sequencing, beta diversity analysis highlighted a significant difference (p = 0.001, n = 10) in the murine microbiota between the four diet groups. The differences between the two nisin containing diets were mainly attributable to differences in the nisin release from the starch matrices while the differences between the carriers were mainly attributable to the type of resistant starch they possessed. Indeed, the differences in the relative abundance of several genera in the mice consuming the starch dough and starch gel diets, in particular Akkermansia, the relative abundance of which was 0.5 and 11.9%, respectively (p = 0.0002, n = 10), points to the potential value of resistance starch as a modulator of beneficial gut microbes. Intact nisin and nisin digestion products (in particular nisin fragment 22-31) were detected in the feces and the nisin was biologically active. However, despite a three-fold greater consumption of nisin in the group fed the nisin in starch dough diet, twice as much nisin was detected in the feces of the group which consumed the nisin in starch gel diet. In addition, the relative abundance of three times as many genera from the lower gastrointestinal tract (GIT) were significantly different (p < 0.001, n = 10) to the control for the group fed the nisin in starch gel diet, implying that the starch gel afforded a degree of protection from digestion to the nisin entrapped within it.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine (10/RD/TMFRC/701); Teagasc (Walsh Fellowship scheme 2012221); Science Foundation Ireland (SFI/16/BBSRC/3389); Biotechnology and Biological Sciences Research Council (BB/P009875/1)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1186
dc.identifier.citationGough, R., Cabrera Rubio, R., O'Connor, P. M., Crispie, F., Brodkorb, A., Miao, S., Hill, C., Ross, R. P., Cotter, P. D., Nilaweera, K. N. and Rea, M. C. (2018) 'Oral Delivery of Nisin in Resistant Starch Based Matrices Alters the Gut Microbiota in Mice', Frontiers in Microbiology, 9, 1186 (15pp). doi: 10.3389/fmicb.2018.01186en
dc.identifier.doi10.3389/fmicb.2018.01186
dc.identifier.endpage15
dc.identifier.issn1664-302X
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/6834
dc.identifier.volume9
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2018.01186/full
dc.rights© 2018, Gough, Cabrera Rubio, O'Connor, Crispie, Brodkorb, Miao, Hill, Ross, Cotter, Nilaweera and Rea. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMouseen
dc.subjectNisinen
dc.subjectStarchen
dc.subjectResistant starchen
dc.subjectMicrobiotaen
dc.subjectDigestionen
dc.subjectAkkermansiaen
dc.subject~Microbiology - Journal Articles~en
dc.titleOral delivery of nisin in resistant starch based matrices alters the gut microbiota in miceen
dc.typeArticle (peer-reviewed)en
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