dc.contributor.author	Bergmann, Silke
dc.contributor.author	Beard, Philippa M.
dc.contributor.author	Pasche, Bastian
dc.contributor.author	Lienenklaus, Stefan
dc.contributor.author	Weiss, Siegfried
dc.contributor.author	Gahan, Cormac G.	en
dc.contributor.author	Schughart, Klaus
dc.contributor.author	Lengeling, Andreas
dc.contributor.funder	National German Genome Network
dc.contributor.funder	European Commission
dc.contributor.funder	Biotechnology and Biological Sciences Research Council
dc.contributor.funder	Helmholtz Centre for Infection Research, Germany
dc.date.accessioned	2016-02-25T17:20:02Z
dc.date.available	2016-02-25T17:20:02Z
dc.date.issued	2013-04-23
dc.description.abstract	Background: The bacterial surface protein internalin (InlA) is a major virulence factor of the food-born pathogen Listeria monocytogenes. It plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule E-cadherin. In mice, the interaction of InlA with murine E-cadherin is impaired due to sequence-specific binding incompatibilities. We have previously used the approach of ‘murinisation’ to establish an oral listeriosis infection model in mice by exchanging two amino acid residues in InlA. This dramatically increases binding to mouse E-cadherin. In the present study, we have used bioluminescent murinised and non-murinised Listeria strains to examine the spatiotemporal dissemination of Listeria in four diverse mouse genetic backgrounds after oral inoculation. Results: The murinised Listeria monocytogenes strain showed enhanced invasiveness and induced more severe infections in all four investigated mouse inbred strains compared to the non-murinised Listeria strain. We identified C57BL/6J mice as being most resistant to orally acquired listeriosis whereas C3HeB/FeJ, A/J and BALB/cJ mice were found to be most susceptible to infection. This was reflected in faster kinetics of Listeria dissemination, higher bacterial loads in internal organs, and elevated serum levels of IL-6, IFN-γ, TNF-α and CCL2 in the susceptible strains as compared to the resistant C57BL/6J strain. Importantly, murinisation of InlA did not cause enhanced invasion of Listeria monocytogenes into the brain. Conclusion: Murinised Listeria are able to efficiently cross the intestinal barrier in mice from diverse genetic backgrounds. However, expression of murinized InlA does not enhance listerial brain invasion suggesting that crossing of the blood brain barrier and crossing of the intestinal epithelium are achieved by Listeria monocytogenes through different molecular mechanisms.	en
dc.description.sponsorship	National German Genome Network (NGFN-Plus Grant Number 01GS0855); European Commission (EUMODIC Project - Framework Programme 6: LSHG-CT-2006-037188; European COST Action ‘SYSGENET’ BM901); Biotechnology and Biological Sciences Research Council, United Kingdom (Institute Strategic Grant); Helmholtz Centre for Infection Research (HZI), Germany (Institute Strategic Grant)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	90
dc.identifier.citation	BERGMANN, S., BEARD, P. M., PASCHE, B., LIENENKLAUS, S., WEISS, S., GAHAN, C. G. M., SCHUGHART, K. & LENGELING, A. 2013. Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice. BMC Microbiology, 13:90, 1-16. http://dx.doi.org/10.1186/1471-2180-13-90	en
dc.identifier.doi	10.1186/1471-2180-13-90
dc.identifier.endpage	16	en
dc.identifier.issn	1471-2180
dc.identifier.journaltitle	BMC Microbiology	en
dc.identifier.startpage	1	en
dc.identifier.uri	https://hdl.handle.net/10468/2403
dc.identifier.volume	13	en
dc.language.iso	en	en
dc.publisher	BioMed Central Ltd.	en
dc.rights	© Bergmann et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.	en
dc.rights.uri	http://creativecommons.org/licenses/by/2.0	en
dc.subject	Central nervous system	en
dc.subject	I interferon receptor	en
dc.subject	Monocytogenes infection	en
dc.subject	E-cadherin	en
dc.subject	Genetic control	en
dc.subject	Salmonella Typhimurium	en
dc.subject	Bacterial infection	en
dc.subject	Intestinal barrier	en
dc.subject	Surface protein	en
dc.subject	Innate immunity	en
dc.subject	~Microbiology - Journal Articles~	en
dc.subject	~Pharmacy - Journal Articles~	en
dc.title	Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice	en
dc.type	Article (peer-reviewed)	en

Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice

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