A click chemistry route to 2-functionalised PEGylated and cationic beta-cyclodextrins: co-formulation opportunities for siRNA delivery

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dc.contributor.authorO'Mahony, Aoife M.
dc.contributor.authorOgier, Julien R.
dc.contributor.authorDesgranges, Stephane
dc.contributor.authorCryan, John F.
dc.contributor.authorDarcy, Raphael
dc.contributor.authorO'Driscoll, Caitríona M.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderIrish Drug Delivery Networken
dc.contributor.funderIrish Research Council for Science, Engineering and Technologyen
dc.date.accessioned2013-01-09T10:31:02Z
dc.date.available2013-01-09T10:31:02Z
dc.date.copyright2012
dc.date.issued2012-05-21
dc.date.updated2013-01-04T15:00:08Z
dc.description.abstractA new approach to the synthesis of amphiphilic beta-cyclodextrins has used 'click' chemistry to selectively modify the secondary 2-hydroxyl group. The resulting extended polar groups can be either polycationic or neutral PEGylated groups and these two amphiphile classes are compatible in dual cyclodextrin formulations for delivery of siRNA. When used alone with an siRNA, a cationic cyclodextrin was shown to have good transfection properties in cell culture. Co-formulation with a PEGylated cyclodextrin altered the physicochemical properties of nanoparticles formed with siRNA. Improved particle properties included lower surface charges and reduced tendency to aggregate. However, as expected, the transfection efficiency of the cationic vector was lowered by co-formulation with the PEGylated cyclodextrin, requiring future surface modification of particles with targeting ligands for effective siRNA delivery.en
dc.description.sponsorshipScience Foundation Ireland (Strategic Research Cluster grant no. 07/SRC/B1154), Irish Research Council for Science, Engineering and Technology (EMBARK initiative)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationO'Mahony, A.M.,Ogier, J.,Desgranges, S.,Cryan, J.F.,Darcy, R.,O'Driscoll, C.M. (2012) 'A click chemistry route to 2-functionalised PEGylated and cationic beta-cyclodextrins: co-formulation opportunities for siRNA delivery'. Organic & Biomolecular Chemistry, 10 :4954-4960. doi: 10.1039/c2ob25490een
dc.identifier.doi10.1039/c2ob25490e
dc.identifier.endpage4960en
dc.identifier.issn1477-0520
dc.identifier.issn1477-0539
dc.identifier.journaltitleOrganic & Biomolecular Chemistryen
dc.identifier.startpage4954en
dc.identifier.urihttps://hdl.handle.net/10468/869
dc.identifier.volume10en
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.rights© The Royal Society of Chemistry 2012en
dc.subjectGene deliveryen
dc.subjectChain-lengthen
dc.subjectNanoparticlesen
dc.subjectParticlesen
dc.subjectVectorsen
dc.subjectTraffickingen
dc.subjectPolyplexesen
dc.subject.lcshCyclodextrinsen
dc.titleA click chemistry route to 2-functionalised PEGylated and cationic beta-cyclodextrins: co-formulation opportunities for siRNA deliveryen
dc.typeArticle (peer-reviewed)en
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