Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide–gold nanoparticle conjugates

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dc.contributor.authorMurphy, Deirdre
dc.contributor.authorEritja, Ramon
dc.contributor.authorRedmond, Gareth
dc.contributor.funderSixth Framework Programme
dc.contributor.funderHigher Education Authority
dc.contributor.funderDirección General de Investigación Científica y Técnica
dc.contributor.funderGeneralitat de Catalunya
dc.date.accessioned2017-11-14T13:24:32Z
dc.date.available2017-11-14T13:24:32Z
dc.date.issued2004
dc.description.abstractGold nanoparticle labels, combined with UV-visible optical absorption spectroscopic methods, are employed to probe the temperature-dependent solution properties of DNA triple helices. By using oligonucleotide-nanoparticle conjugates to characterize triplex denaturation, for the first time triplex to duplex melting transitions may be sensitively monitored, with minimal signal interference from duplex to single strand melting, for both parallel and antiparallel triple helices. Further, the comparative sequence-dependent stability of DNA triple helices may also be examined using this approach. Specifically, triplex to duplex melting transitions for triplexes formed using oligonucleotides that incorporate 8-aminoguanine derivatives were successfully monitored and stabilization of both parallel and antiparallel triplexes following 8-aminoguanine substitutions is demonstrated.en
dc.description.sponsorshipHigher Education Authority (PRTLI1 Nanoscale Science and Technology Initiative); Dirección General de Investigación Científica y Técnica (BQU2000-0649 and BQU2003-00397); Generalitat de Catalunya (2001-SGR-0049)
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide65
dc.identifier.citationMurphy, D., Eritja, R. and Redmond, G. (2004) 'Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide–gold nanoparticle conjugates', Nucleic Acids Research, 32(7), e65 (7pp). doi: 10.1093/nar/gnh065en
dc.identifier.doi10.1093/nar/gnh065
dc.identifier.issn0305-1048
dc.identifier.issued7
dc.identifier.journaltitleNucleic Acids Researchen
dc.identifier.urihttps://hdl.handle.net/10468/5034
dc.identifier.volume32
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.projectinfo:eu-repo/grantAgreement/NIH/NATIONAL CENTER FOR RESEARCH RESOURCES/2M01RR001066-21/US/SFTY &EFFCY OF SUBSTANCE-P ANTAGONIST CJ-11974 &PLBO IN SUBJ W/ULCER COLITIS/
dc.relation.urihttps://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gnh065
dc.rights© 2004, Oxford University Press all rights reserveden
dc.subject3rd stranden
dc.subjectColorimetric detectionen
dc.subjectPurineen
dc.subject8-Amino-2'-deoxyguanosineen
dc.subjectPolynucleotidesen
dc.subjectStabilizationen
dc.subjectParallelen
dc.subjectDuplexen
dc.titleMonitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide–gold nanoparticle conjugatesen
dc.typeArticle (peer-reviewed)en
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